Inhibitory Effect of Carallia Brachiata Extract Through Regulation of Adipogenesis Pathways in 3T3-L1 Cells

Abstract

Background: Pharmacological effects of Carallia brachiata Merr. has been reported to show antioxidant effects against the development of diabetes. However, the mechanism underlying antiadipogenic activity have not been investigated. Objective: Effect of Carallia brachiata ethanolic extract was determined on inhibition of adipogenesis in 3T3-L1 adipocytes. Materials and Methods: Adipose tissue development was performed in preadipocyte 3T3-L1 cells culture. Carallia brachiata leaf (CL) and stem (CS) part were selected for measuring cytotoxicity, accumulation of lipids, and genes involved in adipogenic differentiation. Results: During the adipogenic differentiation, CS down-regulated gene expression of adipogenic transcription factors (PPARγ, C/EBPα, aP2, FAS, LPL and SREBP1c). However, CL only suppressed SREBP1c and aP2 genes. The accumulation of lipids was suppressed by CS, but CL could not show this effect. Conclusion: Our findings suggest that ethanol extract of Carallia brachiata stem has a better anti-adipogenesis effect than the leaf part by suppressing adipogenesis-related gene expression. Moreover, inhibition of lipid storage could be decreased insulin resistance risk. Key words: Carallia brachiata, Adipogenesis, 3T3-L1 adipocytes