A C2 domain containing plasma membrane protein of Plasmodium falciparum merozoites mediates calcium-dependent binding and invasion to host erythrocytes

Abstract

Abstract Background: Invasion of red blood cells by Plasmodium falciparum merozoites is governed by multiple receptoreligand interactions which are critical for bridging the two cells together. The critical function of these ligands for invasion and their direct exposure to the host immune system makes them lucrative vaccine candidates. This necessitates the discovery of new adhesins with less redundancy that mediates the binding of merozoite to the red cell, and furthermore invasion into it. Here we have identified a novel membrane associated antigen (PfC2DMA) that is conserved throughout the Plasmodium species and has a membrane targeting C2 domain at its extreme N-terminal region. Methods: Recombinant C2dom was expressed heterologously in bacteria and purified to homogeneity. Mice antisera against C2dom was raised and used to check the expression and intraparasitic localization of the protein. RBC and Ca2þ ion binding activity of C2dom was also checked. Results: C2dom exhibited specific binding to Ca2þ ions and not to Mg2þ ions. PfC2DMA localized to the surface of merozoite and recombinant C2dom bound to the surface of human RBCs. RBC receptor modification by treatment with different enzymes showed that binding of C2dom to RBC surface is neuraminidase sensitive. Mice antisera raised against C2dom of Pf C2DMA showed invasion inhibitory effects. Conclusion: Our findings suggest that C2dom of PfC2DMA binds to surface of red cell in a Ca2þdependent manner, advocating a plausible role in invasion and can serve as a potential novel blood stage vaccine candidate.