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DC Field | Value | Language |
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dc.contributor.author | Guo, Ming-Quan | - |
dc.contributor.author | Wang, Yi-Ting | - |
dc.contributor.author | Wang, Shan-Shan | - |
dc.contributor.author | dkk. | - |
dc.date.accessioned | 2024-12-14T04:42:05Z | - |
dc.date.available | 2024-12-14T04:42:05Z | - |
dc.date.issued | 2024-02 | - |
dc.identifier.issn | 1684-1182 | - |
dc.identifier.uri | http://localhost:8080/xmlui/handle/123456789/9009 | - |
dc.description.abstract | Background: Hypervirulent carbapenem-resistant Klebsiella pneumoniae (Hv-CRKP) triggered a significant public health challenge. This study explored the prevalence trends and key genetic characteristics of Hv-CRKP in one Shanghai suburbs hospital during 2014e2018. Methods: During five years, Hv-CRKP strains identified from 2579 CRKP by specific PCR, were subjected to performed short- and long-read sequencing technology; epidemiological characteristics, antimicrobial-resistance genes (ARGs), virulence determinants, detailed plasmid profiles and conjugation efficiency were comprehensively investigated. Results: 155 Hv-CRKP and 31 non-Hv-CRKP strains were sequenced. Hv-CRKP strains exhibited significant resistance to six common antibiotic classes (>92%). ST11 steadily increased and became the most prevalent ST (85.2%), followed by ST15 (8.5%), ST65 (2.6%), ST23 (1.9%), and ST86 (0.6%). ST11-KL64 (65.2%) rapidly increased from 0 in 2014 to 93.9% in 2018. blaKPC-2 was the primary carbapenemase gene (97.4%). Other ARGs switched from aac(3)-IId to aadA2 in aminoglycoside and from sul1 to sul2 in sulfanilamide. The time-dated phylogenetic tree was divided into four independent evolutionary clades. Clade 1 and 3 strains were mostly limited in the ICU, whereas Clade 2 strains were distributed among multiple departments. Compared to ybt14 in ICEKp12 in Clade 1, Clade 3 strains harbored ybt9 in ICEKp3 and blaCTX-M-65. Hv-CRKP infected more wards than non-Hv-CRKP and showed greater transmission capacity. Three plasmids containing crucial carbapenemase genes demonstrated their early transmission across China. Conclusion: The Hv-CRKP ST11-KL64 has rapidly replaced ST11-KL47 and emerged as the predominant epidemic subtype in various hospital wards, highlighting the importance of conducting comprehensive early surveillance for Hv-CRKP, especially in respiratory infections. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Journal of Microbiology, Immunology and Infection | en_US |
dc.relation.ispartofseries | Original Article;128-137 | - |
dc.subject | Hypervirulent carbapenemresistant Klebsiella pneumoniae | en_US |
dc.subject | Genomic epidemiology | en_US |
dc.subject | Virulence plasmid | en_US |
dc.subject | Carbapenem resistance gene | en_US |
dc.title | Genomic epidemiology of hypervirulent carbapenem-resistant Klebsiella pneumoniae at Jinshan local hospital, Shanghai, during 2014-2018 | en_US |
dc.type | Article | en_US |
Appears in Collections: | Vol. 57 No. 1 (2024) |
Files in This Item:
File | Description | Size | Format | |
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128-137.pdf | 2.46 MB | Adobe PDF | View/Open |
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