Antigout Activity of Terap (Artocarpus odoratissimus Blanco) Leaves Extract: Leaves Characteristic and In Vitro Studies

Abstract

Terap leaves (Artocarpus odoratissimus Blanco) contain various chemical constituents, making it a herbal medicine prospective source, particularly as an antigout. This research aim to determinate characteristics of terap leaves and in vitro antigout activity of terap leaves ethanolic extract. The determination leaves characteristics included macroscopic and microscopic analysis, water and ethanol soluble extract content, and drying shrinkage. The extract antigout activity was determinated through inhibition of xanthine oxidase enzyme in vitro. Leaves characteristics analysis revealed that mature leaves are approximately 31.5 cm long and 18 cm wide. Terap leaves are single leaves, elliptical, blunt leaf tips, rounded bases, rough surfaces, undulating edges, pinnate veins, and yellow veins. Microscopic analysis reveals the presence of anomocytic-type stomata, trichomes, glandular hairs, resin ducts, and trachea. Terap leaves have a water-soluble extract content of 8.72%, ethanol-soluble extract content of 4.29%, and drying shrinkage of 11.38%. The antigout activity tests results indicates that ethanol extract inhibits xanthine oxidase enzyme with an IC50 of 89.63 μg/mL. Terap leaves have antigout properties, so they have the potential to be developed into a quality antigout herbal medicine. Keywords: Artocarpus odoratissimus, Antigout Activity, Xanthine Oxidase Enzyme, Macroscopic and Microscopic Analysis