The Effect of Omega-3 Rich Fish Oil on the Kidney Changes in Mice Induced by Azoxymethane and Dextran Sodium Sulfate

Abstract

Background: The study aimed to investigate the effect of omega-3 rich fish oil to kidney of mice induced by Azoxymethane (AOM) and DSS using histopathology parameters. Method: The experimental mice were induced using 10 mg/kg AOM and 2% DSS for 2 weeks randomly allocated randomly into four groups as follows; Control Group: mice that not received fish oil, Low Dose Group: mice that received 1.5 mg/day fish oil, Medium Dose Group: mice that received 3 mg/day fish oil, and High Dose Group: mice that received 6 mg/day fish oil. The omega-3 rich fish oil was given for 12 weeks. Result: The administration of high dose omega-3 rich fish oil was able to reduced necrosis and inflammation foci compared to the control group (p<0.05). Furthermore, the administration of low, medium, and high dose omega-3 rich fish oil was able to significantly reduced vascular edema and cell degeneration foci (p<0.05). The administration of medium and high dose of omega-3 rich fish oil were able to reduce the amount of fibrosis foci compared to the control group (p<0.05) compared to the control group. Conclusion: The result suggested anti-nephrotoxic effect of omega-3 rich fish oil in mice induced by azoxymethane and DSS. Key words: Omega-3, Fish oil, Histopathology, Kidney, Mice, Azoxymethane, Dextran sodium sulfate