Antioxidant activity of panJe tea combination of pandan (Pandanus amaryllifolius) and jahe (Zingiber officinale)

Abstract

Herbal tea is popular because of its potential as medicine, a combination of herbal ingredients promises to produce new colors, flavors, and tastes. This study aimed to determine the antioxidant activities of an herbal tea combinations (pandan and jahe) called PanJe and compared it to the antioxidant single herbal tea namely Pandan tea (0.5 g dried pandan) and Ginger tea (2 g dried ginger). The PanJe tea was a combination of 0.5 g pandan tea and 2 g ginger tea. Pandan tea, ginger tea and PanJe tea are soaked for 5 minutes in 200 mL of boiling water. The antioxidant activities were assayed namely 2,2-Diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2), 2,2′-azino-bis(3ethylbenzothiazoline-6-sulfonic acid (ABTS) free radical scavenger, and Ferric Reducing Antioxidant Power (FRAP) assay. The flavonoid content used Quercetin Equivalent (QE), while the total phenol content used Gallic Acid Equivalent (GAE). DPPH, H2O2, ABTS scavenging activities and FRAP assay of PanJe tea was presented in median inhibitory concentration (IC50) value respectively 14.69, 27.70, 4.11 and 7.09%, while pandan tea resulted in IC50 value of 23.63, 27.19, 4.54 and 12.86%, and ginger tea resulted in IC50 value of 7.76, 13.23, 1.26 and 4.94%, respectively. These assays proved ginger tea had the highest antioxidant activities compared to PanJe tea and pandan tea which had the lowest antioxidant activities. Total phenolic and flavonoid of PanJe respectively were 0.035 µg GAE/mg and 0.006 µg QE/mg of PanJe tea meanwhile pandan tea respectively contain 0.002 µg GAE/mg, 0.0003 µg QE/mg of pandan tea and ginger tea contained 0.118 µg GAE/mg and 0.036 QE/mg of ginger tea. Compared to PanJe tea and pandan tea, ginger tea has the highest total phenol and flavonoid content. Conclusion ginger tea is higher antioxidant activities also a higher phenol and flavonoid content than PanJe tea