Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/7899
Title: Analysis of soft gelatin capsule with real-time polymerase chain reaction for halal autenthication
Authors: Salamah, Nina
Guntarti, Any
Nurani, Laela Hayu
Keywords: DNA
halal
porcine gelatin
real-time PCR
soft capsule
Issue Date: Mar-2023
Publisher: Pharmaciana
Abstract: Halal medicine is an interesting topic to always discuss because it is a priority choice for Muslim consumers, one of which is halal capsules. Currently, molecular biology techniques such as real-time polymerase chain reactions are rapidly developing, including for the analysis of non-halal components based on DNA sequences. This study aimed to validate the quantitative PCR method for identifying DNA in gelatin-based products and to apply the confirmation method designed for capsule samples on the market circulating in Yogyakarta to prove the halalness of these samples. Validation of the porcine DNA detection analysis method on standard extraction of porcine gelatin using primer pairs obtained in previous studies. Validated methods are used for testing market capsule shells. The qPCR method using D-loop primers is specifically capable of amplifying porcine gelatin DNA up to a concentration of 0.5 pg/µL, with a CV value in the amplification response of porcine gelatin DNA isolates (1000 pg/µL) of 0.85% which meets the test criteria using the PCR. Three samples of commercial soft capsules tested gave a positive amplification response, meaning that the samples tested contained porcine DNA, and one negative sample, which probably had non-porcine gelatin. The application of this method is also very useful for ensuring the authenticity of the capsule shell, especially from cross-contamination and counterfeiting.
URI: http://localhost:8080/xmlui/handle/123456789/7899
ISSN: 2088 4559
Appears in Collections:VOL 13 NO 1 2023

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