Effect of Thiamine on Serum Glutamate in Ischemic Stroke Animal Model

Abstract

Background: Thiamin or vitamin b1 is a therapy that has been widely used in neurology cases such as alcohol-induced encephalopathy and neuropathy. Thiamin has the ability to penetrate the blood brain barrier so that it is expected to be used as an additional therapy in stroke cases. Stroke is currently an emergency neurology case that causes high mortality and disability. Among the causes is because damage to large areas of the brain will cause the release of neurotransmitters such as glutamate which has excitotoxicity effects to the brain when excessive. Method: This study used animal models of carotid artery occlusion stroke. Divided into 4 groups: control, thiamin dose of 100mg/kg, Thiamin 200mg/kg, and 400mg/kg. Serum glutamate was collected on day 7 post-action. Then evaluated by ELISA method. Results: There was a significant difference in the form of a decrease in the average serum glutamate level between the control and the sample that received a dose of thiamine 200mg/kg and 400mg/kg. Conclusion: High-dose thiamine may be considered as an additional therapy in cases of carotid artery occlusion model stroke. Key words: Thiamine, Glutamate, Animal model, Ischemic, Stroke