Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/9395
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dc.contributor.authorChen, Xiang-Jun-
dc.contributor.authorWan, Tsai-Wen-
dc.contributor.authorChao, Qiuo-Ting-
dc.contributor.authorTeng, Lee-Jene-
dc.contributor.authorLee, Tai-fen-
dc.contributor.authorHuang, Yu-Tsung-
dc.date.accessioned2024-12-20T01:43:56Z-
dc.date.available2024-12-20T01:43:56Z-
dc.date.issued2023-08-
dc.identifier.citationOriginal Articleen_US
dc.identifier.urihttp://localhost:8080/xmlui/handle/123456789/9395-
dc.description.abstractAbstract Background and purpose: Early laboratory identification of group B Streptococcus (GBS, Streptococcus agalactiae) in the birth canal of pregnant women is critical for prompt administration of antimicrobial therapy and may further reduce the mortality rate due to GBS neonatal infection. Methods: A total of 164 vaginal/rectal swab specimens collected from pregnant women at 35 e37 weeks of gestation were screened for GBS vaginal colonization. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS, Bruker Biotyper, Bruker Daltonik GmbH, Bremen, Germany) system was used to detect GBS from Carrot broth and LIM broth enrichment using an in-house extraction protocol. The results were compared to those by conventional broth-enriched culture/identification methods as the gold standard. BD MAX GBS assay (Becton Dickinson, Sparks, MD, USA) was also performed for Carrot brothenriched specimen. Discordant results were investigated using the GeneXpert GBS PCR assay (Cepheid Inc., Sunnyvale, CA, USA). Results: Using the extraction protocol, 33 (20.1%) of the 164 specimens were positive in Carrot broth, and 19 (11.6%) were positive in LIM broth. Using the culture protocol, 38 (23.2%) samples in Carrot broth and 35 (21.3%) in LIM broth were positive. The sensitivity, specificity, and positive and negative predictive values using the extraction protocol in Carrot broth and LIM broth compared to the gold standard conventional culture/identification method were 86.8% and 50.0%, 100% and 100%, 100% and 100%, and 96.2% and 86.9%, respectively. Conclusions: The extraction protocol with MALDI-TOF MS from Carrot broth-enriched samples provides a more rapid turnaround time, lower cost, and acceptable sensitivity and specificity to correctly identify pathogens when compared to conventional culture/identification methods.en_US
dc.language.isoen_USen_US
dc.publisherElsevier Taiwan LLCen_US
dc.subjectStreptococcus agalactiaeen_US
dc.subjectExtractionen_US
dc.subjectMALDI-TOF MSen_US
dc.subjectReal-time PCRen_US
dc.subjectCarrot brothen_US
dc.subjectLIM brothen_US
dc.titleApplicability of an in-house extraction protocol in a Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry system for the identification of Streptococcus agalactiae from broth-enriched vaginal/rectal swab specimensen_US
dc.typeArticleen_US
Appears in Collections:VOL 56 NO 4 2023

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