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DC Field | Value | Language |
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dc.contributor.author | Yu, Shan-Chi | - |
dc.contributor.author | Chen, Ko-Chen | - |
dc.contributor.author | Huang, Ruby Yun-Ju | - |
dc.date.accessioned | 2024-12-19T07:29:49Z | - |
dc.date.available | 2024-12-19T07:29:49Z | - |
dc.date.issued | 2023-08 | - |
dc.identifier.citation | Original Article | en_US |
dc.identifier.uri | http://localhost:8080/xmlui/handle/123456789/9370 | - |
dc.description.abstract | Abstract Background: Reactive lymphadenopathies such as toxoplasmosis and cytomegalovirus lymphadenitis are associated with monocytoid cell proliferation. Monocytoid cells are B-lymphocytes with an undetermined subset. Methods: Using digital spatial profiling whole transcriptome analyses, this study compared monocytoid and control B-cells. The B-cell subset of monocytoid cells was assigned according to gene expression profiles. Results: This study identified 466 differentially expressed genes between monocytoid and control B-cells. The cellular deconvolution algorithm identified monocytoid cells as memory Bcells instead of as naı¨ve B-cells. A comparison of the upregulated genes revealed that atypical memory B-cells had the largest number of genes overlapping with monocytoid cells compared with other memory B-cell subsets. Atypical memory B-cell markers, namely TBX21 (T-bet), FCRL4 (IRTA1), and ITGAX (CD11c), were all upregulated in monocytoid cells. Similar to atypical memory B-cells, monocytoid cells exhibited (1) upregulated transcription factors (TBX21, TOX), (2) upregulated genes associated with B-cell inhibition (FCRL5, FCRL4) and downregulated genes associated with B-cell activation (PIK3CG, NFKB1A, CD40), (3) downregulated cell cycle-related genes (CDK6, MYC), and (4) downregulated cytokine receptors (IL4R). This study also analyzed the expression of monocytoid cell signature genes in various memory B-cell subsets. Atypical memory B-cells exhibited a gene expression pattern similar to that of monocytoid cells, but other memory B-cell subsets did not. Furthermore, monocytoid cells and marginal zone lymphomas differed in gene expression profiles. Conclusion: Spatial transcriptomic analyses indicated that monocytoid cells may be atypical memory B-cells. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier Taiwan LLC | en_US |
dc.subject | Age-associated Bcells | en_US |
dc.subject | Gene expression | en_US |
dc.subject | GeoMx | en_US |
dc.subject | Memory B-cells | en_US |
dc.subject | Monocytoid cells | en_US |
dc.title | Nodal reactive proliferation of monocytoid B-cells may represent atypical memory B-cells | en_US |
dc.type | Article | en_US |
Appears in Collections: | VOL 56 NO 4 2023 |
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File | Description | Size | Format | |
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729-738.pdf | 3.88 MB | Adobe PDF | View/Open |
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