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dc.contributor.authorYu, Shan-Chi-
dc.contributor.authorChen, Ko-Chen-
dc.contributor.authorHuang, Ruby Yun-Ju-
dc.date.accessioned2024-12-19T07:29:49Z-
dc.date.available2024-12-19T07:29:49Z-
dc.date.issued2023-08-
dc.identifier.citationOriginal Articleen_US
dc.identifier.urihttp://localhost:8080/xmlui/handle/123456789/9370-
dc.description.abstractAbstract Background: Reactive lymphadenopathies such as toxoplasmosis and cytomegalovirus lymphadenitis are associated with monocytoid cell proliferation. Monocytoid cells are B-lymphocytes with an undetermined subset. Methods: Using digital spatial profiling whole transcriptome analyses, this study compared monocytoid and control B-cells. The B-cell subset of monocytoid cells was assigned according to gene expression profiles. Results: This study identified 466 differentially expressed genes between monocytoid and control B-cells. The cellular deconvolution algorithm identified monocytoid cells as memory Bcells instead of as naı¨ve B-cells. A comparison of the upregulated genes revealed that atypical memory B-cells had the largest number of genes overlapping with monocytoid cells compared with other memory B-cell subsets. Atypical memory B-cell markers, namely TBX21 (T-bet), FCRL4 (IRTA1), and ITGAX (CD11c), were all upregulated in monocytoid cells. Similar to atypical memory B-cells, monocytoid cells exhibited (1) upregulated transcription factors (TBX21, TOX), (2) upregulated genes associated with B-cell inhibition (FCRL5, FCRL4) and downregulated genes associated with B-cell activation (PIK3CG, NFKB1A, CD40), (3) downregulated cell cycle-related genes (CDK6, MYC), and (4) downregulated cytokine receptors (IL4R). This study also analyzed the expression of monocytoid cell signature genes in various memory B-cell subsets. Atypical memory B-cells exhibited a gene expression pattern similar to that of monocytoid cells, but other memory B-cell subsets did not. Furthermore, monocytoid cells and marginal zone lymphomas differed in gene expression profiles. Conclusion: Spatial transcriptomic analyses indicated that monocytoid cells may be atypical memory B-cells.en_US
dc.language.isoen_USen_US
dc.publisherElsevier Taiwan LLCen_US
dc.subjectAge-associated Bcellsen_US
dc.subjectGene expressionen_US
dc.subjectGeoMxen_US
dc.subjectMemory B-cellsen_US
dc.subjectMonocytoid cellsen_US
dc.titleNodal reactive proliferation of monocytoid B-cells may represent atypical memory B-cellsen_US
dc.typeArticleen_US
Appears in Collections:VOL 56 NO 4 2023

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