A randomized trial of vitamin D supplementation to prevent seasonal influenza and enterovirus infection in children

Abstract

Abstract Purpose: This study aimed to evaluate whether vitamin D supplementation can reduce the incidence of influenza and enterovirus infection in Taiwanese children. Methods: This randomized, double-blind, controlled trial included children aged two to five years between April 2018 and October 2019 from daycare centers. All the participants were randomly assigned to a vitamin D supplementation group (2000 IU/day) or placebo group for one month. The primary outcome was the incidence of influenza and enterovirus infection in the following six months, and the secondary outcome was the incidence of influenza and enterovirus infection in the children’s household members. Results: Two hundred and forty-eight children participated. The vitamin D group showed a relative risk reduction of 84% against influenza compared to the placebo group but did not reach statistical significance. KaplaneMeier curves revealed that the placebo group had a higher probability of influenza infection than the vitamin D group (log-rank test, p Z 0.055), but the incidence of enterovirus infection was similar between the two groups (p Z 0.946) among children. Among children’s household members, the incidence of influenza (p Z 0.586) and enterovirus infection (p Z 0.528) were both similar between the two groups. All childrenwho were tested for serum 25(OH)D levels after vitamin D intervention had 25(OH)D levels above 30 ng/ml Conclusion: Vitamin D supplementation may have a small preventative effect against influenza infection but does not affect enterovirus infection among preschool children. A high-dose short-term vitamin D intervention might be a way to elevate children’s serum vitamin D levels in the first month of starting kindergarten.