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DC Field | Value | Language |
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dc.contributor.author | Li, Yicheng | - |
dc.contributor.author | Wuermanbieke, Shalitanati | - |
dc.contributor.author | Zhang, Xiaogang | - |
dc.contributor.author | Ma, Hairong | - |
dc.contributor.author | Qi, Fei | - |
dc.date.accessioned | 2024-12-19T03:44:52Z | - |
dc.date.available | 2024-12-19T03:44:52Z | - |
dc.date.issued | 2022-08-01 | - |
dc.identifier.issn | 1684-1182 | - |
dc.identifier.uri | http://localhost:8080/xmlui/handle/123456789/9312 | - |
dc.description.abstract | Abstract Background: The D-isoforms of amino acids (D-AAs) exhibit anti-biofilm potential against a diverse range of bacterial species in vitro, while its role in vivo remains unclear. The aim of this study was to investigate the effects of a combination of D-AAs and vancomycin on a PJI rat model. Methods: Eight-week-old male SD rats were randomized to the control group, sham group, vancomycin group, D-AAsevancomycin group. After treatment for 6 weeks, we analysed the levels of inflammatory factors in serum, behavioural change, imaging manifestations. The antibiofilm ability of D-AAs was detected by crystal violet staining and scanning electron microscope observation, and its ability to assist antibiotics in killing bacteria was assessed by culture of bacteria. Additionally, micro-CT and histological analysis were used to evaluate the impact of D-AAs combined with vancomycin on the bone remodelling around the prosthesis. Results: The group treated with a D-AAsevancomycin combination sustained normal weight gain and exhibited reduced the serum levels of a2M, IL-1b, IL-6, IL-10, TNF-a and PGE2. Moreover, treated with D-AAs in combination with vancomycin improved the weight-bearing activity performance, increased the sizes and widths of distal femurs, and improved Rissing scale scoring. In particular, treatment using D-AAs enhanced the ability of vancomycin to eradicate Staphylococcus aureus, as demonstrated by the dispersion of existing biofilms and the inhibition of biofilm formation that occurred in a concentration-dependent manner. This treatmentcombination also resulted in a reduction in bacterial burden with in the soft tissues, bones, and implants. Furthermore, D-AAsevancomycin combination treatment attenuated abnormal bone remodelling around the implant, as evidenced by an observed increase in BMD, BV/TV, and Tb.Th and the presence of reduced Trapþ osteoclasts and elevated osterixþ osteo-progenitors. Conclusions: Combining D-AAs with vancomycin provides an effective therapeutic strategy for the treatment of PJI by promoting biofilm dispersion to enhance antimicrobial activity | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier Taiwan LLC | en_US |
dc.subject | D-amino acids; | en_US |
dc.subject | Vancomycin; | en_US |
dc.subject | Periprosthetic joint infection | en_US |
dc.title | Effects of intra-articular D-amino acids combined with systemic vancomycin on an experimental Staphylococcus aureusinduced periprosthetic joint infection | en_US |
dc.type | Article | en_US |
Appears in Collections: | VOL 55 NO 4 2022 |
Files in This Item:
File | Description | Size | Format | |
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716-727.pdf | 3.3 MB | Adobe PDF | View/Open |
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