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DC Field | Value | Language |
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dc.contributor.author | Kuo, Chih-Hao | - |
dc.contributor.author | Lin, Wei-Feng | - |
dc.contributor.author | Liu | - |
dc.contributor.author | Wang, Zhe-Chong | - |
dc.contributor.author | Liu, Ting-Yi | - |
dc.contributor.author | Peng, Hwei-Ling | - |
dc.date.accessioned | 2024-12-19T03:44:22Z | - |
dc.date.available | 2024-12-19T03:44:22Z | - |
dc.date.issued | 2023-06 | - |
dc.identifier.citation | Original Article | en_US |
dc.identifier.uri | http://localhost:8080/xmlui/handle/123456789/9311 | - |
dc.description.abstract | Abstract Background: CpxAR is a two-component system that allows bacteria to reorganize envelope structures in response to extracellular stimuli. CpxAR negatively affects type 1 fimbriae expression in Klebsiella pneumoniae CG43, a hypervirulent strain. The involvement of CpxAR in the regulation of type 3 fimbriae expression was investigated. Methods: cpxAR, cpxA, and cpxR gene-specific deletion mutants were generated. The deletion effects on the expression of type 1 and type 3 fimbriae were analyzed via measuring the promoter activity, mannose sensitive yeast agglutination activity, biofilm formation, and the production of the major pilins FimA and MrkA respectively. RNA sequencing analysis of CG43S3, DcpxAR, DcpxR and Dfur was employed to study the regulatory mechanism influencing the expression of type 3 fimbriae. Results: Deletion of cpxAR increased type 1 and type 3 fimbrial expression. Comparative transcriptomic analysis showed that the expression of oxidative stress-responsive enzymes, type 1 and type 3 fimbriae, and iron acquisition and homeostasis control systems were differentially affected by cpxAR or cpxR deletion. Subsequent analysis revealed that the small RNA RyhB negatively affects the expression of type 3 fimbriae, while CpxAR positively controls ryhB expression. Finally, the site-directed mutation of the predicted interacting sequences of RyhB with the mRNA of MrkA attenuated the RyhB repression of type 3 fimbriae. Conclusion: CpxAR negatively regulates the expression of type 3 fimbriae by modulating cellular iron levels thereafter activating the expression of RyhB. The activated RyhB represses the expression of type 3 fimbriae by base-pairing binding to the 50 region of mrkA mRNA | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier Taiwan LLC | en_US |
dc.subject | CpxAR twocomponent system | en_US |
dc.subject | Iron homeostasis control | en_US |
dc.subject | Klebsiella pneumoniae CG43 | en_US |
dc.subject | Small RNA RyhB | en_US |
dc.subject | Type 3 fimbriae expression | en_US |
dc.title | Role of the stress-responsive two-component system CpxAR in regulating fimbriae expression in Klebsiella pneumoniae CG43 | en_US |
dc.type | Article | en_US |
Appears in Collections: | VOL 56 NO 3 2023 |
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File | Description | Size | Format | |
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464-476.pdf | 1.78 MB | Adobe PDF | View/Open |
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