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DC Field | Value | Language |
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dc.contributor.author | -Feng Lin, Wei | - |
dc.contributor.author | -Yu Hu, Rong | - |
dc.contributor.author | -You Chang, Hwan | - |
dc.contributor.author | -Yu Lin, Fang | - |
dc.contributor.author | -Hao Kuo, Chih | - |
dc.contributor.author | -Hsin Su, Li | - |
dc.contributor.author | Ling Peng, Hwei- | - |
dc.date.accessioned | 2024-12-19T02:33:34Z | - |
dc.date.available | 2024-12-19T02:33:34Z | - |
dc.date.issued | 2022-08-01 | - |
dc.identifier.issn | 1684-1182 | - |
dc.identifier.uri | http://localhost:8080/xmlui/handle/123456789/9270 | - |
dc.description.abstract | Abstract Background/purpose: Two urease operons were identified in Klebsiella pneumoniae CG43, ure-1 and ure-2. This study investigates whether a differential regulation of the expression of ure-1 and ure-2 exists and how urease activity influences the acid stress response and expression of type 1 and type 3 fimbriae. Methods: The ureA1 and ureA2 gene specific deletion mutants were constructed. Promoter activity was assessed using a LacZ reporter system. The sensitivity to acid stress was determined by assessing the survival after pH 2.5 treatment. The influence on type 1 and type 3 fimbriae expression was assessed using western blotting and mannose-sensitive yeast agglutination and biofilm formation assay, respectively. Results: Bacterial growth analysis in mM9-U or modified Stuart broth revealed that ure-1 was the principal urease system, and ure-2 had a negative effect on ure-1 activity. Deletion of the fur or nac gene had no apparent effect on the activity of Pure1, Pure2-1, and Pure2-2. The Pure2-2 activity was enhanced by deletion of the hns gene. ureA1 deletion increased acid stress sensitivity, whereas the deleting effect of ureA2 was notable without hns. Deletion of ureA1 or ureA2 significantly induced the expression of type 1 fimbriae but decreased MrkA production and biofilm formation. Conclusion: ure-1 is the primary expression system in K. pneumoniae CG43, while ure-2 is active in the absence of hns. Impairment of urease activity increases the sensitivity to acidstress, and the accumulation of urea induces the expression of type 1 fimbriae but represses type 3 fimbriae expression | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier Taiwan LLC | en_US |
dc.subject | Klebsiella pneumoniae CG43; | en_US |
dc.subject | Ure-1 and Ure-2 operons; | en_US |
dc.subject | Acid stress response; | en_US |
dc.subject | Type 1 and type 3 fimbriae | en_US |
dc.title | The role of urease in the acid stress response and fimbriae expression in Klebsiella pneumoniae CG43 | en_US |
dc.type | Article | en_US |
Appears in Collections: | VOL 55 NO 4 2022 |
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File | Description | Size | Format | |
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620-633.pdf | 2.19 MB | Adobe PDF | View/Open |
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