Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/9263
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dc.contributor.authorLi, Wei-You-
dc.contributor.authorYin, Shuhui-
dc.contributor.authorHuang, Szu-Wei-
dc.contributor.authorYang, Ming-Hui-
dc.contributor.authorChen, Patricia MT.-
dc.contributor.authorWu, Shang-Rung-
dc.date.accessioned2024-12-19T02:24:33Z-
dc.date.available2024-12-19T02:24:33Z-
dc.date.issued2023-04-
dc.identifier.citationOriginal Articleen_US
dc.identifier.urihttp://localhost:8080/xmlui/handle/123456789/9263-
dc.description.abstractAbstract Background: The exploration of virology knowledge was limited by the optical technology for the observation of virus. Previously, a three-dimensional multi-resolution real-time microscope system (3D-MRM) was developed to observe the uptake of HIV-1-tat peptide-modified nanoparticles in cell membrane. In this study, we labeled HIV-1 virus-like particles (VLPs) with passivated giant quantum dots (gQDs) and recorded their interactive trajectories with human Jurkat CD4 cells through 3D-MRM. Methods: The labeled of gQDs of the HIV-1 VLPs in sucrose-gradient purified viral lysates was first confirmed by Cryo-electronic microscopy and Western blot assay. After the infection with CD4 cells, the gQD-labeled VLPs were visualized and their extracellular and intracellular trajectories were recorded by 3D-MRM. Results: A total of 208 prime trajectories was identified and classified into three distinct patterns: cell-free random diffusion pattern, directional movement pattern and cell-associated movement pattern, with distributions and mean durations were 72.6%/87.6 s, 9.1%/ 402.7 s and 18.3%/68.7 s, respectively. Further analysis of the spatialetemporal relationship between VLP trajectories and CD4 cells revealed the three stages of interactions: (1) cell-associated (extracellular) diffusion stage, (2) cell membrane surfing stage and (3) intracellular directional movement stage. Conclusion: A complete trajectory of HIV-1 VLP interacting with CD4 cells was presented in animation. This encapsulating method could increase the accuracy for the observation of HIV-1- CD4 cell interaction in real time and three dimensions.en_US
dc.language.isoen_USen_US
dc.publisherElsevier Taiwan LLCen_US
dc.subjectHIV-1en_US
dc.subjectQuantum dotsen_US
dc.subjectSingle virus trackingen_US
dc.subjectThree-dimensional multi-resolution microscopeen_US
dc.subjectVirus-like particleen_US
dc.titleThe trajectory patterns of single HIV-1 viruslike particle in live CD4 cells: A real time three-dimensional multi-resolution microscopy study using encapsulated nonblinking giant quantum doten_US
dc.typeArticleen_US
Appears in Collections:VOL 56 NO 2 2023

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