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DC Field | Value | Language |
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dc.contributor.author | Lee, Tai-fen | - |
dc.contributor.author | Lin, Kuan-Yin | - |
dc.contributor.author | Chang, Sui-Yuan | - |
dc.contributor.author | Huang, Yu-Tsung | - |
dc.contributor.author | Hsueh, Po-Ren | - |
dc.date.accessioned | 2024-12-17T02:34:19Z | - |
dc.date.available | 2024-12-17T02:34:19Z | - |
dc.date.issued | 2024-02 | - |
dc.identifier.citation | Original Article | en_US |
dc.identifier.uri | http://localhost:8080/xmlui/handle/123456789/9123 | - |
dc.description.abstract | Abstract Background and purpose: This study aimed to investigate the etiologies of sexually transmitted infections (STIs) among men who have sex with men (MSM) in Taiwan. Methods: Two commercial assays, the BD MAX Chlamydia trachomatis (CT), Neisseria gonorrhoeae (GC), and Trichomonas vaginalis (TV) panel and the Allplex STI Essential assay (CT, GC, Mycoplasma genitalium [MG], Mycoplasma hominis [MH], Ureaplasma urealyticum [UU], Ureaplasma parvum [UP], and TV) were evaluated. During the first stage, urine and rectal swab samples from 168 patients were evaluated using the BD MAX assay, and the multiplex RT-PCR Allplex STI Essential assay was applied only to the patients with positive results on the BD MAX asay (n Z 49). During the second stage, urine and rectal swab samples from 90 patients were evaluated using the BD MAX assay and the Allplex qPCR. Results: The Allplex qPCR identified all CT, missed one and additionally one TV from the positive samples (n Z 49) by the BD MAX assay in the first stage. At the second stage, both commercial assays showed similar detection rate of CT, NG or CT/NG coinfection (11.1%, 1.1% and 4.4% by the BD MAX assay; 10.0%, 1.1% and 2.2% by the Allplex qPCR). The positivity rates of MG, MH, and UU by the Allplex qPCR were 4.4%, 2.2%, and 12.2%, respectively, for urine samples and 10%, 13.3%, and 22.2%, respectively, for anal swab samples. Conclusions: High rates of STI-associated etiologies were observed in MSM. The positive rateswere higher in rectal swabs than in urine samples. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Journal of Microbiologi, Immunology and Infection | en_US |
dc.subject | Sexually transmitted infections | en_US |
dc.subject | Allplex STI Essential assay | en_US |
dc.subject | BD MAX assay | en_US |
dc.subject | Performance | en_US |
dc.subject | Men who have sex with men | en_US |
dc.title | Performance of two commercial multiplex polymerase chain reaction assays for the etiological diagnosis of sexually transmitted infections among men who have sex with men | en_US |
dc.type | Article | en_US |
Appears in Collections: | VOL 56 NO 1 2023 |
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104-110.pdf | 288.67 kB | Adobe PDF | View/Open |
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