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DC Field | Value | Language |
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dc.contributor.author | Chen, Pek Kee | - |
dc.contributor.author | Lee, Yi-Tzu | - |
dc.contributor.author | Liu, Chia-Ying | - |
dc.contributor.author | dkk. | - |
dc.date.accessioned | 2024-12-14T06:40:09Z | - |
dc.date.available | 2024-12-14T06:40:09Z | - |
dc.date.issued | 2024-04 | - |
dc.identifier.issn | 1684-1182 | - |
dc.identifier.uri | http://localhost:8080/xmlui/handle/123456789/9044 | - |
dc.description.abstract | Background: This study aimed to characterize carbapenem-nonsusceptible Acinetobacter (CNSA) isolated from patients with bacteremia from 1997 to 2015. Methods: A total of 173 CNSA (12.3%) was recovered from 1403 Acinetobacter isolates. The presence of selected b-lactamase genes in CNSA was determined by PCR amplification. The conjugation test was used to determine the transferability of metallo-b-lactamase (MBL)-carrying plasmids. Whole genome sequencing in combination with phenotypic assays was carried out to characterize MBL-plasmids. Results: In general, a trend of increasing numbers of CNSA was observed. Among the 173 CNSA, A. baumannii (54.9%) was the most common species, followed by A. nosocomialis (23.1%) and A. soli (12.1%). A total of 49 (28.3%) CNSA were extensively drug-resistant, and all were A. baumannii. The most common class D carbapenemase gene in 173 CNSA was blaOXA-24-like (32.4%), followed by ISAba1-blaOXA-51-like (20.8%), ISAba1-blaOXA-23 (20.2%), and IS1006/IS1008-blaOXA-58 (11.6%). MBL genes, blaVIM-11, blaIMP-1, and blaIMP-19 were detected in 9 (5.2%), 20 (11.6%), and 1 (0.6%) CNSA isolates, respectively. Transfer of MBL genes to AB218 and AN254 recipient cells was successful for 7 and 6 of the 30 MBL-plasmids, respectively. The seven AB218-derived transconjugants carrying MBL-plasmids produced less biofilm but showed higher virulence to larvae than recipient AB218. Conclusions: Our 19-year longitudinal study revealed a stable increase in CNSA during 2005 e2015. blaOXA-24-like, ISAba1-blaOXA-51-like, and ISAba1-blaOXA-23 were the major determinants of Acinetobacter carbapenem resistance. MBL-carrying plasmids contribute not only to the carbapenem resistance but also to A. baumannii virulence. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Journal of Microbiology, Immunology and Infection | en_US |
dc.relation.ispartofseries | Original Article;288-299 | - |
dc.subject | Acinetobacter spp. | en_US |
dc.subject | Bacteremia | en_US |
dc.subject | blaVIM | en_US |
dc.subject | blaIMP | en_US |
dc.subject | Carbapenem resistance | en_US |
dc.subject | Carbapenemase | en_US |
dc.subject | Conjugative plasmid | en_US |
dc.subject | Virulence | en_US |
dc.title | A 19-year longitudinal study to characterize carbapenem-nonsusceptible Acinetobacter isolated from patients with bloodstream infections and the contribution of conjugative plasmids to carbapenem resistance and virulence | en_US |
dc.type | Article | en_US |
Appears in Collections: | Vol. 57 No. 2 (2024) |
Files in This Item:
File | Description | Size | Format | |
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288-299.pdf | 1.82 MB | Adobe PDF | View/Open |
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