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DC Field | Value | Language |
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dc.contributor.author | Hung, Shih-Yuan | - |
dc.contributor.author | Yeh, Yuan-Ming | - |
dc.contributor.author | Chiu, Cheng-Hsun | - |
dc.contributor.author | dkk. | - |
dc.date.accessioned | 2024-12-14T04:54:22Z | - |
dc.date.available | 2024-12-14T04:54:22Z | - |
dc.date.issued | 2024-02 | - |
dc.identifier.issn | 1684-1182 | - |
dc.identifier.uri | http://localhost:8080/xmlui/handle/123456789/9015 | - |
dc.description.abstract | Background: Proper identification of the polymicrobial microorganisms in patients with limb-threatening diabetic foot ulcers (LTDFUs) using conventional culture is insufficient. This prospective study evaluates the potential value of adjuvant molecular testing assisting in identify fastidious micro-organisms in LTDFUs compared to standard treatment alone. Methods: Ninety patients with LTDFUs received interdisciplinary and standard antibiotic treatment in a referral diabetic foot center. A simultaneous 16S amplicon sequencing (16S AS) specimen along with conventional culture collected at admission was used to retrospectively evaluate the microbiological findings and its association with amputation outcomes. Results: The microorganism count revealed by 16S AS overwhelmed that of conventional culturing (17 vs. 3 bacteria/ulcer respectively). The Stenotrophomonas spp. revealed in 29 patients were highly correlated with major (above ankle) amputation (OR: 4.76, 95% CI 1.01 e22.56), while only one had been concomitantly identified by conventional culturing. Thus, there were 27 cases without proper antibiotics coverage during treatment. Conclusions: Adjuvant molecular testing assisted identification of fastidious pathogens such as Stenotrophomonas infection and might be associated with major amputation in patients with LTDFUs. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Journal of Microbiology, Immunology and Infection | en_US |
dc.relation.ispartofseries | Original Article;156-163 | - |
dc.subject | Limb-threatening DFU | en_US |
dc.subject | Wound culture | en_US |
dc.subject | 16S amplicon sequencing | en_US |
dc.subject | Fastidious pathogens | en_US |
dc.subject | Stenotrophomonas spp | en_US |
dc.title | Microbiome of limb-threatening diabetic foot ulcers indicates the association of fastidious Stenotrophomonas and major amputation | en_US |
dc.type | Article | en_US |
Appears in Collections: | Vol. 57 No. 1 (2024) |
Files in This Item:
File | Description | Size | Format | |
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156-163.pdf | 1.14 MB | Adobe PDF | View/Open |
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