Effect of Tender Coconut Water (TCW) on TNF-α, IL-1 and IL-6 in Streptozotocin (STZ) and Nicotinamid (NA) Induced Diabetic Rats

Abstract

Background: Diabetes Mellitus (DM) is characterized by an increase in blood sugar levels also known as hyperglycemia. Continuous hyperglycemia can increase the production of Reactive Oxygen Species (ROS). ROS causes oxidative stress and increases the formation of TNF-α expression as a marker of inflammation. Tender coconut water is rich in sources of free amino acids, L-arginine and vitamin C which can prevent oxidative stress. Aim and Objectives: This research to investigate the effect of tender coconut water on TNF-α, IL-1 and IL-6 in Streptozotocin (STZ) and Nicotinamid (NA) induced diabetic rats. Material and Methods: Experimental research design using posttest control group design. Twenty four male wistar strain rats were used in this study were divided randomly into 4 groups, which are group K1 (control); K2 (DM type 2); K3 (DM type 2+ Glibenclamid 0,18mg/200grBW); K4 (DM type 2+ tender coconut water 8mL/200gr BW). Type 2 Diabetes Mellitus were induced using Streptozotocin (STZ) 65mg/kg body weight and Nicotinamid 230 mg/kg body weight. The administration of tender coconut water were given on day 3 after DM condition is reached, given daily for 4 weeks with dose of 8 mL/200 gr BW. Data on of TNF-α , IL-1 and IL-6 levels were analyzed by One Way Anova. Result: Average TNF-α level, IL-1 level and IL-6 level in Group 2 increased compared to Group 1 , in Group 3 it decreased compared to Group 2 as well as in Group 4 .The results of the analysis has the p values <0.05. Conclusion: Administration of tender coconut water can be decreasing of TNF-α, IL-1 and IL-6 levels in wistar strain male rats with type 2 Diabetes Mellitus. Key words: Diabetes mellitus, Tender coconut water, TNF-α, IL-1, IL-6.