Extract of Torbangun (Coleus amboinicus Lour) Leaves Inhibits the Expression of PEPCK and G6Pase in Streptozotocin-Induced Diabetic Rats

Abstract

Objective: This study aimed to analyze the anti-hyperglycemic activity of Torbangun leaf extract (TLE) in hyperglycemic rats by observing its effect on blood glucose levels and PEPCK and G6Pase gene expression. Method: This post-test-controlled group design study used a completely randomized design (CRD). Seven rats were separated as a normal group (N) and other rats were injected with streptozotocin (STZ). Hyperglycemia was characterized by fasting blood glucose (FBG) >126 mg/dl. Hyperglycemic rats were divided into three groups: H-IM (control drug metformin hydrochloride dose 62.5 mg/kg BW), H-IT (TLE dose 620mg/kg BB), and NG (negative control, hyperglycemic rats). Rats were given intervention for 14 days. Result: The results showed a significant difference between initial and final FBG of the HI-IM group (p = 0.002, α = 0.01) and the H-IT group (p = 0.005, α = 0.01) and effect on the key gene expression of gluconeogenesis in the liver. The H-IT group showed a decrease in PEPCK gene expression 0.80 times and decreased G6Pase gene expression by 0.65 times compared to NG group. Conclusion: The findings suggested the administration of TLE in STZ-induced hyperglycemic rats was able to reduce blood sugar levels and was involved in inhibiting the expression of PEPCK and G6Pase expression genes in the liver. Key words: G6Pase, Hyperglycemia, PEPCK, Rats, Torbangun