HPTLC Comparision Studies with Marker Compound and Isolation of Rutin Anti Inflammatory Agent from Ethanolic Leaf Extract of Rivea Hypocrateriformis (Desr.) Choisy

Abstract

Introduction: To extract the leafy parts with ethanol and perform the HPTLC studies with marker compounds Quercetin, Rutin, and gallic acid then a compound was isolated and susceptible to spectroscopic analysis Background: Rivea hypocrateriformis (Desr.) Choisy holds Convolvulaceae family the leafy parts underwent a Soxhlet extraction with ethanol which was then subjected to a phytochemical investigation that shows the presence of alkaloids, phenols, flavonoids, terpenoids, tannins, glycosides saponins, sterols, and carbohydrates. Material and methods: Firstly, the ethanolic leaf extract is treated with petroleum ether to remove excess lipids, sterols, and impurities. TLC was performed by toluene: ethyl acetate: formic acid and the same solvent system was used for column chromatography. The bands getting separated into the column were collected separately in a test tube. Once the solvent toluene ends, the next solvent system with 18 ml of toluene and 2 ml of ethyl acetate, is introduced into the column, and continues the separation procedure. Collect separated bands and perform TLC of each separate band by using the same solvent system in a concentration of 5:4:1. A single component on a TLC plate with a solvent system of 12 ml toluene and 8 ml ethyl acetate was obtained whose characterization was done by IR, NMR, and mass. Results: The RF value of Rutin is 0.17 which correlates with the Retention factor of ethanolic leaf extracts of RH by HPTLC comparative studies and a compound was isolated by column chromatography. Conclusion: These studies have concluded that a compound is separated by means of HPTLC and spectroscopic interpretation and identified as Rutin. Key words: HPTLC studies, Marker compound, Column chromatography, Rivea hypocrateriformis ethanolic leaf extract, TLC plates