Association of rs121912724 polymorphism in Apolipoprotein A1 gene with diabetic dyslipidemia and correlation with serum High-density lipoprotein Cholesterol, Triglycerides and Low-density lipoprotein Cholesterol levels

Abstract

Objectives: To identify the association of the rs121912724 polymorphism in the Apolipoprotein A1 gene with diabetic dyslipidemia and its correlation with serum High-density Lipoprotein Cholesterol (HDL), Triglycerides, (TG) and Low-density Lipoprotein Cholesterol (LDL-C) levels. Methods: Two groups were taken, comprising 150 diabetic dyslipidemia (group I) patients and 150 healthy controls (group II). Demographic and biochemical data were analyzed using Statistical Package for Social Sciences 26 by applying the Student independent t-test. DNA samples of both groups were subjected to TETRA-Amplification Refractory System polymerase chain reaction, and allele A and C of the rs121912724 polymorphism were amplified. The association of the rs121912724 polymorphism with the disease was studied using Fisher's exact test and Cochran-Armitage test. The correlation between the polymorphism and levels of lipid levels was determined using Pearson correlation on SPSS version 27. Results: The levels of HDL-C, LDL-C, and TG were significantly higher than in healthy group (p<0.000). The genotypic count of homozygous AA was 137, 12 of heterozygous AC, and 1 of homozygous CC in group I. The genotypic count of homozygous AA was 138, 12 of heterozygous AC in group II, with no homozygous CC. There was no association of rs121912724 observed with the development of diabetic dyslipidemia, and a negative correlation of rs121912724 with deranged levels of HDL-C, LDL-C, and TG was observed. Conclusion: The rs121912724 polymorphism in the APOA1 gene was not associated with diabetic dyslipidemia. There was no correlation found between the polymorphism and deranged levels of HDL-C, TG, and LDL-C.