Potential of Rhinachanthus nasutus (L.) Kurz Leaves Extract as an Antioxidant and Inhibitor of α-Glucosidase Activity

Abstract

Aims: The goal of this study is to learn more about the antioxidant and antidiabetic properties of Rhinachantus nasutus (L.) Kurz (RnLK) leaf extract. The Ultrasound-Assisted Extraction (UAE) technique was used to extract the leaf material, and the solvent used was ethanol with a 70% concentration. The total phenol content (TPC) of the extracted material was determined. The Cupric Ion Reducing Antioxidant Capacity (CUPRAC) method was used to examine antioxidant activity, whereas α-glucosidase activity was used to test antidiabetic action. Results: The ethanol extract of RnLK leaves yielded 8.36%, with a TPC of 607.1±0.2 mg GAE/g sample. The IC50 value for leaf extract antioxidant activity was 19.1±0.1 mg/L. Furthermore, the leaf extract inhibits α-glucosidase activity and has an IC50 value of 81.3±3 mg/L, making it an antidiabetic. Conclusion: The ethanolic extract of RnLK leaves can be used as an alternative antioxidant and antidiabetic material, according to the findings of this study. Key words: RnLK, UAE, CUPRAC method, Anti-diabetic, α-glucosidase activity.