The Effect of Antioxidant activity, Total Phenols and Total Flavonoids on Arginase Inhibitory Activity on Plants of Genus Sterculia

Abstract

Background: The genus of Sterculia has the main compound of phenol and flavonoids. The secondary metabolites which have an arginase inhibitory activities were phenol and flavonoids. The aim of this study was to investigate the arginase inhibitory activity from genus Sterculia. The Plant of Sterculia: Sterculia rubiginosa Zoll. ex Miq., Sterculia comosa (Wall) Roxb., Sterculia parkinsonii F. Muell, Sterculia macrophylla Vent, Sterculia Stipulata Korth. The simplisia were leaves and woods. Materials and Methods: The simplisia were extracted with n-hexane, ethyl acetate and methanol. The ethyl acetate and methanol extract determined the arginase inhibition activity. The active extracts as an arginase inhibitory, determined the total flavonoids, total phenols and antioxidant activity, and the chemical content. Sterculia comosa (Wall) Roxb., Sterculia macrophylla Vent, Sterculia Stipulata Korth., have arginase inhibitory activity. Results: The ethyl acetate extracts of Sterculia Stipulata leaves is an active extract. The methanol extract which have an arginase inhibitor activity were Sterculia comosa (Wall) Roxb. wood and leaves, Sterculia macrophylla Vent., wood and leaves, Sterculia stipulata Korth., wood, and leaves. The methanol extract of Sterculia comosa (Wall) Roxb. Woods has the highest content of total phenols, antioxidant activity, and arginase inhibitory activity. The methanol extract of Sterculia macrophylla Vent. has the highest content of total flavonoids, but this extract as an arginase inhibitory activity more lower than Sterculia comosa. The active extract as an arginase activity was methanol extract of Sterculia comosa (Wall) Roxb. Conclusion: The total phenols were more contributed for the response of the arginase inhibitory activity much more than antioxidant activity and total flavonoids. Key words: Arginase, Antioxidant, Enzyme, Flavonoids, Phenols, Sterculia