Ethanol-based solvent system for recovery antioxidant activity Centella asiatica L. Urban and its application in sleep-deprived Rats

Abstract

Antioxidants can eliminate free radicals by donating electrons to oxidants. Preclinical and clinical studies show that Centella asiatica L. Urban has antioxidant activity. Ethanol was the best solvent for extracting antioxidant compounds from Centella asiatica L. Urban. The concentration of ethanol solvents affects the compounds extracted and their antioxidant activities. The study used sleep-deprived rats with glutathione (GSH) concentration parameters to identify ethanol-based solvent systems for the optimum recovery of antioxidant activities and the in vivo antioxidant activity of the most active extract. C. asiatica L. Urban powder was macerated with 30, 50, 70, and 96% ethanol, respectively. Using a phosphomolybdate reagent, the extract's antioxidant activity was assessed. In vivo, antioxidant activity was tested on the extract with the highest in vitro antioxidant activity. Ethanol-based solvent systems show different recovery antioxidant activities of C. asiatica L. Urban. The antioxidant activity was most recovered in 70% ethanol, with an IC50 value of 76.76±25.29 µg/mL. Ethanol 70% C. asiatica L. Urban extract given to sleep-deprived rats at 300 mg/kg was not significantly different in glutathione levels from those group rats that received the positive control solution (vitamin C). The C. asiatica L. Urban 70% ethanol extract can increase glutathione levels in sleep-deprived rats.