Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/7946
Title: Antibacterial activities of Elaeocarpus ganitrus Roxb. leaves from Kebumen against Escherichia coli and Shigella flexneri
Authors: Kiromah, Naelaz Zukhruf Wakhidatul
Fitriyati, Laeli
Septiani, Sinta Wahyu
Keywords: Elaeocarpus ganitrus Roxb
Escherichia coli
Shigella flexneri
disc diffusion
Issue Date: Jul-2023
Publisher: Pharmaciana
Abstract: Ganitri (Elaeocarpus ganitrus Roxb) is a plant known for its antibacterial properties, particularly in its leaves. The leaves of Ganitri contain antibacterial compounds such as flavonoids, alkaloids, and tannins. The objective of this study is to investigate the antibacterial activity and determine the minimum concentration of ethanol, methanol, and distilled water extracts from Ganitri leaves in inhibiting the growth of Escherichia coli and Shigella flexneri bacteria. A preliminary study of phytochemical with Thin Layer Chromatogram (TLC) and antibacterial study was conducted. The study was conducted in vitro by disc diffusion method with positive control of ciprofloxacin 50 µg/mL and negative control of sterile aquadest. The extract concentration series used were 10, 20, 30, 40, 50, and 100%. The extracts of ganitri leaves have antibacterial activities against Escherichia coli and Shigella flexneri bacteria. The TLC results were observed under visible light at 254 and 365 nm. Ethanol, methanol, and aquadest extracts of ganitri leaves produce spots that are almost comparable to the compounds of quercetin and tannic acid. The inhibitory zone range of the extracts in Escherichia coli bacteria is 10.33-19.66 mm with a strong category. In comparison, the inhibitory zone range of Shigella flexneri is 8.88-22.62 mm with a moderate-very strong category. ANOVA test results stated a significance value of p = 0.001 (p <0.05). Therefore, it can be concluded that the ethanol, methanol, and aquadest extracts of ganitri leaves had significant differences. The minimum concentration of each extract in inhibiting the bacteria Escherichia coli and Shigella flexneri is 10%.
URI: http://localhost:8080/xmlui/handle/123456789/7946
ISSN: 2088 4559
Appears in Collections:VOL 13 NO 2 2023

Files in This Item:
File Description SizeFormat 
232-239.pdf283.25 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.