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Title: | Hepatoprotective Effect of Ganoderma applanatum Crude Polysaccharides on Carbon Tetrachloride-Induced Early Liver Fibrosis in Mice |
Authors: | Joko Kuncoroningrat Susilo, Raden Winarni, Dwi Akhmad Husen, Saikhu Hayaza, Suhailah Puji Astuti Wahyuningsih, Sri Doong, Ruey-An Darmanto, Win |
Keywords: | Anti-inflammatory, Carbon tetrachloride, Crude polysaccharides, Fibrosis, Ganoderma applanatum |
Issue Date: | 2021 |
Abstract: | Introduction: Liver fibrosis is a treatable disease when still at early stages. Ganoderma applanatum plays role as alternative medicine. The fungi have antioxidant, anti-inflammatory, and anticancer bioactivities. This study aimed to evaluate the hepatoprotective effects of G. applanatum crude polysaccharides (GACP) on liver fibrosis due to CCl4 induction. Methods: This study was divided into six groups. BALB/c mice were given CCl4 dissolved in olive oil (2 mL/kg; 1:3) intraperitoneally (i.p) twice a week for four weeks to produce a liver fibrosis model. Distilled water (control group), silymarin 100 mg/kg and GACP 25, 50, 100 mg/kg were given once daily for four weeks. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis-α (TNF-α) and interleukin-6 (IL-6) were determined by enzyme-linked immunosorbent assay (ELISA) kit. Histopathology was stained by hematoxylin & eosin (H&E) and Masson’s trichome. Results: The administration of GACP effectively prevented ALT, AST, TNF-α, and IL-6 levels from high elevation. Additionally, the GACP had protective effect after liver histological analysis exhibited less injury in the liver tissue. Conclusion: The hepatoprotective effect of GACP on liver fibrosis is mainly due to avoid high elevation of pro-inflammatory cytokine. This result revealed that GACP could be a potential hepatoprotective agent for later clinical therapy. Key words: Anti-inflammatory, Carbon tetrachloride, Crude polysaccharides, Fibrosis, Ganoderma applanatum. |
URI: | http://localhost:8080/xmlui/handle/123456789/7903 |
Appears in Collections: | VOL 13 NO 6 2021 |
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