Please use this identifier to cite or link to this item:
http://localhost:8080/xmlui/handle/123456789/7824
Title: | Standardization Extracts and Simplicia of Limau Sundai Peel (Citrus x aurantiifolia 'sundai'), Determine Content of Nobiletin and Antibacterial Activity Test |
Authors: | Husni, Elidahanum Ismed, Friardi Awaliana, Awaliana |
Keywords: | Standardization Sundai lime peel fruit Citrus x aurantiifolia('sundai') Nobiletin TLC Densitometry Antibacterial activity |
Issue Date: | 2021 |
Abstract: | Introduction: One type of oranges typical of West Sumatra, which is widely used as traditional medicine is sundai lime (Citrus x aurantiifolia 'sundai'); Aims: therefore, it is necessary to standardize extracts and Simplicia, determine the content of nobiletin, and antibacterial activity test. Methods: The standardization method was used refer to Farmakope Herbal Indonesia. TLC Densitometry was used to determine the content of the nobiletin, and the diffusion method to antibacterial activity test. To get a good standardization, the sundai lime was taken from three regions: Bukittinggi, Pariaman, and Solok. Results: From these three regions, conclusions drawn from the macroscopic fruit peel slices were uneven and had distinctive odors. The outer surface is brown, and the inner surface is yellowish-white. From the microscopic was identified fragments in sundai lime peels consisting of hair covering, ladder-shaped transport, parenchyma with secretion cells, oxalate crystals, and parenchyma tissue and stomata. Water-soluble extract content of Simplicia ≤ 24.90 %, and ethanol-soluble extract content ≤ 17.66 %. Non-specific parameters are loss on drying ≤ 5.65 %, total ash content ≤ 5.14 %, and acid insoluble ash content ≤ 0.80 %. The specific parameters were crude extract, black, characteristic odor, Rf of nobiletin was 0.75. Rendement extract ≥ 18.80 %. Non-specific parameters of extract were water content ≤ 18.37 %, total ash content ≤ 3.93 %, and non-acidic ash content ≤ 0.27 %. The nobiletin content in the sundai lime extract Pariaman was 0.33 %, Solok 0.59 %, and Bukittinggi 0.47 %. The antibacterial test with diffusion method in three regions has moderate activities as concentrations of 20% and 15%. Conclusion: Sundai lime had Antibacterial activity. Key words: Standardization, Sundai lime peel fruit, Citrus x aurantiifolia('sundai'), Nobiletin, TLC Densitometry, Antibacterial activity. |
URI: | http://localhost:8080/xmlui/handle/123456789/7824 |
Appears in Collections: | VOL 13 NO 6 2021 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.