Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/7751
Title: Characterization of uropathogenic E. coli from various geographical locations in India
Authors: Kumar, Gulshan
Kumar, Yashwant
Kumar, Gaurav
Tahlan, Ajay K.
Keywords: ESBL
MDR
UPEC
Virulence factors
Issue Date: 2023
Publisher: Journal of Taibah University Medical Sciences
Series/Report no.: Original Article;1527-1535
Abstract: Objectives: Uropathogenic Escherichia coli (UPEC) is the most common causative agent of urinary tract infection, accounting for more than 80% of cases worldwide. This study presents data on prevalent serotypes, resistance profiles, and colonization-aiding virulence characteristics of UPEC from different geographical regions in India. Methods: UPEC were serotyped through microtiter plate agglutination. Standard techniques were used to detect various virulence characteristics, i.e., biofilm formation (tissue culture plate method), siderophore production (screened on Chrome Azurol S agar and categorized with Csaky’s and Arnow’s methods), colicin release (agar overlay technique), gelatin hydrolysis (on gelatinase agar), and cell surface hydrophobicity (salt aggregation method). Antibiotic resistance profiles (against 20 antimicrobial agents) and extended-spectrum beta-lactamase (ESBL) were evaluated according to Clinical and Laboratory Standards Institute guidelines. Results: UPEC strains exhibited very high drug resistance rates to most of the commonly used antimicrobial agents; the highest resistance rates were observed for ampicillin (63.4%), nalidixic acid (63.4%), and cefotaxime (62.1%). High rates of multi-drug resistance (63.36%), ESBL-production (34.1%), and carbapenemresistance (25.0%) were detected in UPEC strains from all geographical regions of India. Hydrophobicity (61.2%), biofilm production (62.5%), and siderophore production (67.7%) were the most common virulence characteristics of UPEC isolates. Co-expression of virulence characteristics was common (69.8%) in UPEC strains. Conclusion: UPEC strains with very high antimicrobialresistance are in circulation in India, and have diverse serotypes and virulence characteristics.
URI: http://localhost:8080/xmlui/handle/123456789/7751
ISSN: 1658-3612
Appears in Collections:Vol 18 No 6 (2023)

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