Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/7750
Title: hWJMSCs inhibit inflammation and apoptosis in an ARDS cell model
Authors: Widowati, Wahyu
Wargasetia, Teresa L.
Rahardja, Fanny
Gunanegara, Rimonta F.
Priyandoko, Didik
Gondokesumo, Marisca E.
Novianto, Agung
Yati, Afif
Rizal, Rizal
Keywords: Apoptosis
ARDS
hWJMSCs
Inflammation
NFkB
Issue Date: 2023
Publisher: Journal of Taibah University Medical Sciences
Series/Report no.: Original Article;1519-1526
Abstract: Acute respiratory distress syndrome (ARDS) is a type of lung failure caused by fluids and hypoxemia. Mesenchymal stem cells (MSCs) have been shown to decrease levels of pro-inflammatory mediators and inflammatory cells. These cells have anti-inflammatory, anti-apoptotic, and anti-microbial activity, and protect against lung injury. Objective: This research evaluated the potential of human Wharton’s jelly MSCs (hWJMSCs) to inhibit inflammation and apoptosis in lipopolysaccharide (LPS)- induced rat lung cells (L2). Methods: hWJMSC treatment in LPS-induced rat lung cells was performed with 1:1, 1:5, 1:10, or 1:25 ratios of hWJMSCs to L2 cells. The gene expression of angiotensin-converting enzyme-2 (ACE-2), receptor for advanced glycation end products (RAGE), nuclear factor kappa B (NFkB), and C-X-C motif chemokine ligand-9 (CXCL-9) was quantified with RT-PCR, and the levels of C-reactive protein (CRP), interleukin-12 (IL-12), and tumor necrosis factor-alpha (TNF-a) were measured with ELISA. Results: hWJMSCs increased ACE-2 gene expression, and decreased CXCL-9, NFkB, and RAGE gene expression. The treatment also suppressed CRP, TNF-a, and IL-12 levels, and increased the percentage of live cells, but decreased the percentages of necrotic cells and apoptotic cells in inflammatory rat lung cells, which served as an ARDS cell model. Conclusion: Co-culture of hWJMSCs and L2 cells mitigated inflammation through increasing ACE-2 gene expression, and decreasing CXCL-9, NFkB, and RAGE gene expression; decreasing TNF-a and CRP protein levels; and decreasing necrosis, and early and late apoptosis. A co-culture ratio of 1:1 was most effective.
URI: http://localhost:8080/xmlui/handle/123456789/7750
ISSN: 1658-3612
Appears in Collections:Vol 18 No 6 (2023)

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