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Title: | hWJMSCs inhibit inflammation and apoptosis in an ARDS cell model |
Authors: | Widowati, Wahyu Wargasetia, Teresa L. Rahardja, Fanny Gunanegara, Rimonta F. Priyandoko, Didik Gondokesumo, Marisca E. Novianto, Agung Yati, Afif Rizal, Rizal |
Keywords: | Apoptosis ARDS hWJMSCs Inflammation NFkB |
Issue Date: | 2023 |
Publisher: | Journal of Taibah University Medical Sciences |
Series/Report no.: | Original Article;1519-1526 |
Abstract: | Acute respiratory distress syndrome (ARDS) is a type of lung failure caused by fluids and hypoxemia. Mesenchymal stem cells (MSCs) have been shown to decrease levels of pro-inflammatory mediators and inflammatory cells. These cells have anti-inflammatory, anti-apoptotic, and anti-microbial activity, and protect against lung injury. Objective: This research evaluated the potential of human Wharton’s jelly MSCs (hWJMSCs) to inhibit inflammation and apoptosis in lipopolysaccharide (LPS)- induced rat lung cells (L2). Methods: hWJMSC treatment in LPS-induced rat lung cells was performed with 1:1, 1:5, 1:10, or 1:25 ratios of hWJMSCs to L2 cells. The gene expression of angiotensin-converting enzyme-2 (ACE-2), receptor for advanced glycation end products (RAGE), nuclear factor kappa B (NFkB), and C-X-C motif chemokine ligand-9 (CXCL-9) was quantified with RT-PCR, and the levels of C-reactive protein (CRP), interleukin-12 (IL-12), and tumor necrosis factor-alpha (TNF-a) were measured with ELISA. Results: hWJMSCs increased ACE-2 gene expression, and decreased CXCL-9, NFkB, and RAGE gene expression. The treatment also suppressed CRP, TNF-a, and IL-12 levels, and increased the percentage of live cells, but decreased the percentages of necrotic cells and apoptotic cells in inflammatory rat lung cells, which served as an ARDS cell model. Conclusion: Co-culture of hWJMSCs and L2 cells mitigated inflammation through increasing ACE-2 gene expression, and decreasing CXCL-9, NFkB, and RAGE gene expression; decreasing TNF-a and CRP protein levels; and decreasing necrosis, and early and late apoptosis. A co-culture ratio of 1:1 was most effective. |
URI: | http://localhost:8080/xmlui/handle/123456789/7750 |
ISSN: | 1658-3612 |
Appears in Collections: | Vol 18 No 6 (2023) |
Files in This Item:
File | Description | Size | Format | |
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1519-1526.pdf | 1.98 MB | Adobe PDF | View/Open |
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