Ethanol Extract Activity of Pegagan Embun (Hydrocotyle sibthorpioides L.) Against Hematopoietic on Anemic Male White Mice

Abstract

Introduction: Pegagan embun (Hydrocotyle sibthorpioides L.) is known to have many benefits, including as a blood booster. This study aims to determine the activity of pegagan embun (Hydrocotyle sibthorpioides L.) on the formation of red blood cells. The parameters include the number of erythrocytes, reticulocytes, hemoglobin levels, and hematocrit values in anemic male white mice induced by chloramphenicol 130 mg/kg for 14 days. Methods: Anemic mice consisted of 4 groups, namely the first group was given physiological NaCl, the second, third, and fourth groups were given ethanol extract of pegagan embun (Hydrocotyle sibthorpioides L.) at a dose of 10, 50 and 100 mg/kgper oral every day, for 14 days. Observations were made on days 0, 14, 21, and 28. Results: The results showed that administration of pegagan embun extracts at a dose of 10, 50, and 100 mg/kgto anemic male white mice significantly increase the number of erythrocyte cells, the number of reticulocytes, the hemoglobin levels, and the hematocrit values (p <0.05). Increasing the dose and duration of pegagan embun extract administration could provide a more significant increase in the hematopoietic parameters (p <0.05). Conclusion:It can be concluded that the extract of pegagan embun (Hydrocotyle sibthorpioides L.) can be used to treat anemia in male white mice. Key words: Extract of Hydrocotyle sibthorpioides L., Erythrocytes, Reticulocytes, Hemoglobin, Hematocrit.