Antibacterial Effectiveness of Morinda Citrifolia L. Extract on Salmonella Typhi Bacteria Using Serial Dilution Method with 15 - 60 Minutes Contact Time

Abstract

Noni fruit (Morinda citrifolia L.) contains phenol and flavonoid compounds which can function as natural antibacterials with the highest phenol content obtained from ripe Morinda citrifolia L. fruit. Phenolic compounds have been widely studied as disinfectants against broadspectrum antibacterials, namely Gram-positive and Gram-negative bacteria. This study aims to determine the effectiveness of the inhibitory power of ripe Morinda citrifolia L. extract against the growth of gastroenteritis-causing bacteria with the serial dilution method at concentrations of 40%, 60%, 80%, and 100% with variations in a contact time of 15 ', 30', 45 'and 60'. Then proceed with a concentration of 10%, 20%, 30%, and 40% with the same variation in contact time. The results showed that all concentrations of ripe Morinda citrifolia L. extract and the variation in contact time could inhibit the growth of S.Typhi bacteria with a minimum inhibitory concentration at a concentration of 10% with a contact time of 15 minutes. It needs to be continued with a toxicity test before it is used in the community. Key words: Morinda citrifolia L., Serial dilution, S. Typhi.