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Title: | Antimalarial Potential of Fraction 5 from Ethanolic Leaves Extract of Artocarpus Altilis |
Authors: | Kemalahayati, Einstenia Ilmi, Hilkatul Rosmalina Hidayati, Agriana Wijayanti, Marsih Tumewu, Lidya Suciati, Suciati Fuad Hafid, Achmad Widyawaruyanti, Aty |
Keywords: | antimalaria, Artocarpus altilis, flavonoid, lactate dehydrogenase (LDH), PfMQO |
Issue Date: | 2-Aug-2023 |
Publisher: | Faculty of Pharmacy Univesrsitas Airlangga |
Abstract: | Abstract Background: Artocarpus altilis leaf extract (AAL.E) was separated by VLC, and six fractions were obtained. Fraction 5 (AAL.E.5) showed antimalarial activity with an IC50 value of 3.71 µg/mL. Objective: This study aimed to determine the antimalarial activity of AAL.E.5 subfractions against P. falciparum, the mechanism of action against Plasmodium Falciparum Malate quinone oxidoreductase (PfMQO), and the active substances. Methods: The AAL.E.5 was separated by open-column chromatography and eluted with chloroform-methanol gradient elution in order of increasing polarity. The antimalarial activity of all subfractions was assessed using a lactate dehydrogenase (LDH) assay against P. falciparum and the mechanism of action of the PfMQO enzyme. The profiles of the most active subfractions were analyzed using High-Performance Liquid Chromatography (HPLC). Results: The separation of fraction 5 (AAL.E.5) yielded 11 subfractions (AAL.E.5.1–AAL.E.5.11). Screening antimalarial activity at 10 μg/mL in this subfraction showed that only five subfractions (AAL.E.5.6-AAL. E.5.10) inhibited P. falciparum and two subfractions (AAL.E.5.6 and AAL.E.5.10) inhibited the PfMQO enzyme. Only subfraction 6 (AAL.E.5.6) inhibited both, with IC50 values of 6.609 µg/mL and 20.34 µg/mL. The thin layer chromatography profile of AAL.E.5.6 revealed reddish-orange spots, indicating the presence of flavonoid compounds, and was also presumed from the UV-visible to HPLC chromatogram for band I in the 300 – 400 nm range and band II in the 240–285 nm range. Conclusion: Subfraction 6 has antimalarial activity against P. falciparum and is thought to have a mechanism of action in PfMQO. Based on the TLC, HPLC, and UV-Vis spectra, subfraction 6 was assumed to be a flavonoid. Keywords: antimalaria, Artocarpus altilis, flavonoid, lactate dehydrogenase (LDH), PfMQO |
URI: | http://localhost:8080/xmlui/handle/123456789/7558 |
ISSN: | 25808303 |
Appears in Collections: | VOL 10 NO 2 2023 |
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File | Description | Size | Format | |
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184-192.pdf | 793.77 kB | Adobe PDF | View/Open |
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