Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/7470
Title: Potential Inhibition by Phaleria macrocarpa Leaves Ethanol Extract on Ki-67 Expression in Distal Colon Mouse
Authors: Kusmardi, Kusmardi
Wiyarta, Elvan
Estuningtyas, Ari
Sahar, Nurhuda
Hanafi Midoen, Yurnadi
Tedjo, Aryo
Pakpahan, Alfred
Keywords: Mahkota Dewa (Phaleria macrocarpa), Inflamation
Dextran sodium sulphate,
Issue Date: 2021
Abstract: Ulcerative colitis (UC) has been an important aspect of an incurable chronic inflammatory disease over the last few decades. To find useful therapies for UC, one of which is herbal therapy, many researches have been conducted. Due to its anti-inflammatory effects, Phaleria macrocarpa (PM), an Indonesian indigenous herb, is considered to be the alternative therapy for UC. Phaleria macrocarpa Leaves Ethanol Extract (PMLEE) is then used in this research to determine its effect on UC by using Ki-67 as a marker of proliferation. PMLEE was created from dry PM content undergoing maceration. The animals were classified into six categories: normal, positive control, negative control and PMLEE group (100, 200, 300 mg/kgBW). PMLEE was then injected for 7 consecutive days into BALB/c mice that were caused by dextran sodium sulphate (DSS). DSS is used for modeling UC in the colon tissue of mice. All mice were terminated and then stained with anti-Ki-67 after their colons were extracted. Subsequently, the stained parts were analyzed with ImageJ based on the color intensity produced by the results of H-score. Based on H-score, PMLEE 300mg and 200mg has significantly decreased the expression of Ki-67 compare to the negative control (p=0.001 and p=0.01). PMLEE also has a tendency to be dose dependent based on the significant difference from PMLEE 300mg and 100mg (p=0.002). It then concludes that PMLEE is related to Ki-67 expression in cells, as it was inversely proportional in this analysis. Key words: Mahkota Dewa (Phaleria macrocarpa), Inflamation, Dextran sodium sulphate, Ki- 67.
URI: http://localhost:8080/xmlui/handle/123456789/7470
Appears in Collections:VOL 13 NO 2 2021

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