Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/7433
Title: In Vitro Cytotoxicity and Apoptosis-inducing Activity of Quercus infectoria Extracts in HeLa Cells
Authors: Ismail, Illyana
Suppian, Rapeah
Mohamad, Habsah
Aisha Mohd Radzi, Siti
Abdullah, Hasmah
Keywords: Quercus infectoria
HeLa cells
Cytotoxicity
Apoptosis
Cell cycle
Issue Date: 2021
Abstract: Background: Quercus infectoria galls (QI) extracts were previously reported to have cytotoxicity effects towards human cervical cancer cells, HeLa. However, the underlying molecular mechanisms of the extracts have been poorly determined. Objective: The present study was undertaken to examine the effect of ethyl acetate extracts of QI (EAQI) on cell cytotoxicity and induction of apoptosis in HeLa cells. Materials and Method: The in vitro cytotoxicity was investigated by using the MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] assay and the OD values were read at 570 nm. Meanwhile the induction of apoptosis was measured by using acridine orange and propidium iodide (AO/PI) staining, flow cytometry analysis of annexin V/PI staining and cell cycle distribution. Results: MTT assay showed that EAQI exhibited cytotoxicity effect on HeLa cells with IC50 of 11.50 ± 0.50 μg/ml. HeLa cells underwent apoptosis in response to EAQI treatment, demonstrated by an increase in the percentage of apoptotic cell stained with AOPI from 1.00% to 10.33% compared to untreated cell population (p<0.05) at 72 hours of treatment. The evidence of early apoptosis in treated cells were also observed in annexin V/PI staining. Furthermore, an increase of cell population in sub G0/G1 phase revealed that apoptosis as the mode of cell death in HeLa cells treated with EAQI. Conclusion: These findings indicated that EAQI significantly inhibits HeLa cell growth through induction of apoptosis. Further studies are needed to confirm the mechanism of cell death by expression of apoptotic cascade in HeLa cells treated with EAQI. Key words: Quercus infectoria, HeLa cells, Cytotoxicity, Apoptosis, Cell cycle.
URI: http://localhost:8080/xmlui/handle/123456789/7433
Appears in Collections:VOL 13 NO 2 2021

Files in This Item:
File Description SizeFormat 
13.pdf1.27 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.