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DC Field | Value | Language |
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dc.contributor.author | Chouni, Anirban | - |
dc.contributor.author | Pal, Amrita | - |
dc.contributor.author | K Gopal, Priya | - |
dc.contributor.author | Paul, Santanu | - |
dc.date.accessioned | 2024-11-07T02:26:15Z | - |
dc.date.available | 2024-11-07T02:26:15Z | - |
dc.date.issued | 2021 | - |
dc.identifier.uri | http://localhost:8080/xmlui/handle/123456789/7394 | - |
dc.description.abstract | Introduction: Garcinia cowa (Clusiaceae) is popular among integrative medicine in several communities. This study undertook to evaluate the anti-proliferative activity on cancer cells and its cytotoxic effect on normal cells. Here we are reporting for the first time the metabolomic profiling of G. cowa leaf. Methods: Anti-proliferative potential of ethyl acetate and methanol extract of Garcinia cowa leaf assessed by MTT assay. Metabolomic profiling obtained by GC/ MS analysis. Nuclear morphology visualized by DAPI staining. Caspase activation analysed through spectrophotometric assay. Results: The study reveals, that the methanolic extract is more potential in inducing anti-proliferative activity than ethyl acetate extract. Robust antiproliferative activity of the methanolic extract evidenced in lung cancer cell line, A549 followed by MCF–7, HepG2, MOLT – 4, MDA-MB-468 cells. The anti-proliferative effect was negligible in normal PBMC. Further, a dose-dependent increase of nuclear fragmentation visualized in A549 cells treated with the methanolic extract. Post methanolic extract treatment upregulation of caspase-3 and caspase-9 also evidenced in A549 cells. GC/MS analysis revealed the presence of phytoconstituents of different phytochemical groups comprising of 3.45% diterpenoid, 5.45% triterpenoid, 11.24% steroid, 2.03% phytosterol, etc. in methanol extract, as well as 4.53% diterpenoid, 2.88% triterpenoid, 1.09% steroid, 2.11% phytosterol, etc. in ethyl acetate extract with considerable biological importance. Conclusion: This is the maiden report of the metabolomic profiling of leaf extracts of Garcinia cowa which possess a good repository of potentially bioactive molecules that holds a great promise as a future therapeutic agent in combating lung cancer. Key words: Garcinia cowa, Cancer, GC-MS, Metabolomic profiling, Anti-proliferative. | en_US |
dc.subject | Garcinia cowa | en_US |
dc.subject | Cancer | en_US |
dc.subject | GC-MS | en_US |
dc.subject | Metabolomic profiling | en_US |
dc.subject | Anti-proliferative | en_US |
dc.title | GC-MS Analysis and Screening of Anti-Proliferative Potential of Methanolic Extract of Garcinia cowa on Different Cancer Cell Lines | en_US |
dc.type | Article | en_US |
Appears in Collections: | VOL 13 NO 2 2021 |
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