Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/7394
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dc.contributor.authorChouni, Anirban-
dc.contributor.authorPal, Amrita-
dc.contributor.authorK Gopal, Priya-
dc.contributor.authorPaul, Santanu-
dc.date.accessioned2024-11-07T02:26:15Z-
dc.date.available2024-11-07T02:26:15Z-
dc.date.issued2021-
dc.identifier.urihttp://localhost:8080/xmlui/handle/123456789/7394-
dc.description.abstractIntroduction: Garcinia cowa (Clusiaceae) is popular among integrative medicine in several communities. This study undertook to evaluate the anti-proliferative activity on cancer cells and its cytotoxic effect on normal cells. Here we are reporting for the first time the metabolomic profiling of G. cowa leaf. Methods: Anti-proliferative potential of ethyl acetate and methanol extract of Garcinia cowa leaf assessed by MTT assay. Metabolomic profiling obtained by GC/ MS analysis. Nuclear morphology visualized by DAPI staining. Caspase activation analysed through spectrophotometric assay. Results: The study reveals, that the methanolic extract is more potential in inducing anti-proliferative activity than ethyl acetate extract. Robust antiproliferative activity of the methanolic extract evidenced in lung cancer cell line, A549 followed by MCF–7, HepG2, MOLT – 4, MDA-MB-468 cells. The anti-proliferative effect was negligible in normal PBMC. Further, a dose-dependent increase of nuclear fragmentation visualized in A549 cells treated with the methanolic extract. Post methanolic extract treatment upregulation of caspase-3 and caspase-9 also evidenced in A549 cells. GC/MS analysis revealed the presence of phytoconstituents of different phytochemical groups comprising of 3.45% diterpenoid, 5.45% triterpenoid, 11.24% steroid, 2.03% phytosterol, etc. in methanol extract, as well as 4.53% diterpenoid, 2.88% triterpenoid, 1.09% steroid, 2.11% phytosterol, etc. in ethyl acetate extract with considerable biological importance. Conclusion: This is the maiden report of the metabolomic profiling of leaf extracts of Garcinia cowa which possess a good repository of potentially bioactive molecules that holds a great promise as a future therapeutic agent in combating lung cancer. Key words: Garcinia cowa, Cancer, GC-MS, Metabolomic profiling, Anti-proliferative.en_US
dc.subjectGarcinia cowaen_US
dc.subjectCanceren_US
dc.subjectGC-MSen_US
dc.subjectMetabolomic profilingen_US
dc.subjectAnti-proliferativeen_US
dc.titleGC-MS Analysis and Screening of Anti-Proliferative Potential of Methanolic Extract of Garcinia cowa on Different Cancer Cell Linesen_US
dc.typeArticleen_US
Appears in Collections:VOL 13 NO 2 2021

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