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Title: | Antioxidant and Cytotoxic Bioassay on Blumeodendron toxbrai (Blume.) Stem Bark Hexane, Dichloromethane, and Methanolic Ekstract |
Authors: | Susilo Adi, Andreas Elya, Berna Hanafi, Muhammad |
Keywords: | Blumeodendron toksbraii Cancer Antioxidant Anticytotoxic DPPH MCF-7 |
Issue Date: | 2021 |
Abstract: | Introduction Blumeodendron toksbraii has the potential to be anti-HIV and anti α-glucosidase . Objective: This research was conducted to examine the effects of antioxidant and cytotoxicity in vitro from these compounds from methanolic stem bark extract. Method: Stem bark to be extracted with maceration using hexane, dichloromethane, and methanol solution. Extracts were quantified with respect to in vitro antioxidant activity using the 2.2-diphenyl-1- picrylhydrazyl (DPPH) radical scavenging. Anticytotoxic activity was determined by cytotoxicity assay using MCF-7 cell line with Alamar Blue method. Results: The observed IC50 value from hexane, dichloromethane, and methanol extract for antioxidant assay were 88.33 ± 0.19 μg/ mL, 74,54 ± 0,61 μg /mL and 94.1 ± 0.19 μg/mL respectively. IC50 value of anti-cytotoxic assay from hexane extract, dichloromethane and methanol extract is 121.24 ± 0.15 μg/mL, 55 ± 0,48 μg/mL and 70.71 ± 0.15 μg/mL. Conclusion: dichloromethane extract showed good promising result for anti-oxidant and cytotoxic assay, futher study needed to isolate compound from this plant. Key Words: Blumeodendron toksbraii, Cancer, Antioxidant, Anticytotoxic DPPH, MCF-7. |
URI: | http://localhost:8080/xmlui/handle/123456789/7233 |
Appears in Collections: | VOL 13 NO 1 2021 |
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