Review: Single Chain Fragment Variable sebagai Pemandu Diagnosis dan Terapi Bertarget Human Epidermal Growth Factor Receptor 2 Kanker Payudara

Abstract

Single chain fragment variable (scFv) is a recombinant antibody consisting of an antigen-binding (Fab) antibody fragment with a very small size of about 26 -27 kDa. In general, scFv is stable, encoded by a single gene so that it will facilitate genetic modification. Compared with complete antibodies, scFv which has similar antigen binding activity has other advantages such as small size, better penetration into blood vessels and tissues, and lower immunogenicity. Therefore, scFv has a very good potential to be used as a guide compound for diagnosis and therapy of disease. The results of various studies show that scFv antiHER2 (Human Epidermal Growth Factor Receptor 2) can be used as a guide in the diagnosis of HER2+ breast cancer using either Magnetic Resonance Imaging (MRI) or positron-emission tomography (PET). In HER2 receptor-targeted breast cancer therapy, anti-HER2 scFv also has the potential to be used as a guide for immunotoxins, antibody-drug conjugate (ADC), prodrugs, and pretargeted radioimmunotherapy (PRIT)