Efektivitas Sari Buah Lemon (Citrus limon (L.) Burm. f. sebagai Khelating Agent Logam Berat Tembaga

Abstract

Abstract Background: Lemon (Citrus limon (L.) Burm. F is a fruit that contains citric acid. Citric acid has the ability to bind heavy metals, one of which is copper heavy metals. Objective: The purpose of this study was to determine the effectiveness of lemon juice in reducing copper heavy metals. Phytochemical screening test was carried out to determine the content of secondary metabolites contained in lemon juice. Methods: Determination of the reduction in copper heavy metal levels was carried out by squeezing lemon added 10 ppm simulated copper metal which was read by the Atomic Absorption Spectrophotometer (AAS) method with a wavelength of 324.8 nm. Determination of the reduction in copper metal was carried out using a series of lemon juice concentrations of 10, 20, 30, 40, 50, 60 and 70%. Results: The results showed that lemon juice (Citrus limon (L.) Burm. F. Contains metabolites in the form of citric acid, alkaloids, saponins, and steroids. The average value of reduction in copper metal levels after administration of lemon juice 10% concentration is 0.69 (± 0.42%), 20% concentration of 1.80 (± 0.42%), 30% concentration of 11.85 (± 1.28%), 40% concentration of 33.57 (± 0.33%), 50% concentration was 49.92 (± 0.86%), 60% concentration was 54.14 (± 0.69%) and 70% concentration was 64.89 (± 0.30%). Conclusion: Effectiveness of lemon juice concentration which was able to reduce copper levels by 50% (EC50) was 55.88%. Keywords: lemon juice, EC50 value, copper metal reduction, AAS method