Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/7196
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dc.contributor.authorHermanto, Sandra-
dc.contributor.authorAhmad, Syadzwina Nurdzakiyyah-
dc.contributor.authorRudiana, Tarso-
dc.date.accessioned2024-11-01T03:06:18Z-
dc.date.available2024-11-01T03:06:18Z-
dc.date.issued2023-04-
dc.identifier.citationOriginal Articleen_US
dc.identifier.urihttp://localhost:8080/xmlui/handle/123456789/7196-
dc.description.abstractSoybean is a source of vegetable protein that is rich in nutrients and a source of producing anticancer bioactive peptides. This study aims to determine the optimum conditions for hydrolysis of soybean protein with papain enzyme and their cytotoxicity against the MCF-7 cells. Soybean protein was isolated using an acid precipitation technique. Then, the protein isolate was hydrolyzed using papain enzyme with variations of papain concentration 0,5; 1; and 5% (v/v) and variations of incubation time 0, 1, 2, 3, and 4 hours at 50 °C. The hydrolysates were tested for their degree of hydrolysis (%DH), molecular weight profile using SDS-PAGE, and cytotoxicity against the MCF-7 cells through an in-vitro assay. The most active hydrolysate was fractionated using Sephadex G-15 and characterized by the molecular weight by LCMS/MS. The result showed that the optimum condition for hydrolysis was 1% (v/v) of enzyme concentration and 3 hours of incubation time with a %DH value of 3.01%. Based on the SDSPAGE result, the hydrolysate had protein bands in a lower range (<25 kDa). That hydrolysate has cytotoxicity with an IC50 value of 1.87 mg/mL, and the molecular weight of its bioactive peptide is 7.70 kDa.en_US
dc.language.isoen_USen_US
dc.publisherJurnal Sains Farmasi & Klinisen_US
dc.subjectbioactive peptideen_US
dc.subjectcytotoxicityen_US
dc.subjectMCF-7 cellsen_US
dc.subjectpapainen_US
dc.subjectsoybeanen_US
dc.titleOptimization of Soy Protein Hydrolysis with Papain Enzyme and Its Cytotoxic Activity Against MCF-7 Cancer Cellsen_US
dc.typeArticleen_US
Appears in Collections:VOL 10 NO 1 2023

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