KLT-Bioautografi Ekstrak Etil Asetat Supernatan Hasil Fermentasi Streptomyces G Isolat Tanah Rumah Kompos Bratang Surabaya

Abstract

Abstract Background: The rapid development of microorganisms behavior through various mechanisms reflected in various resistant strains, tolerant and persistent characters, such as Multi Drug Resistant (MDR), Extended Strain Beta Lactamase, MDR-Tuberculosis and Methicillin Resistant Staphylococcus Aureus. Efforts to explore new antibiotics from various natural sources have been done to overcome problems related to the need for antibiotics that are responsible for handling infectious diseases. Objectives: This research focuses on the isolation of Streptomyces sp. from compost waste land in the Bratang area of Surabaya that were able to produce anti-bacterial compounds. Methods: The Streptomyces sp. was successfully isolated and identified as Streptomyces G by which fermentation process was performed in the International Streptomyces Project-4 media. Thin Layer Chromatography-Bioautography method was used to evaluate the antibacterial activity of ethyl acetat extract of cell free fermentation broth (CFFB) against Escherichia coli ATCC 7890 and Staphylococcus aureus ATCC 23456. Results:The CFFB supernatant exhibited ability to inhibit the tested bacteria growth. The active compound was successfully extracted from the supernatant by ethyl acetate. The TLC-bioautogram using eluent of butanol : acetic acid : water (3: 2: 6, v/v) showed that two spots were good separately, one of those (Rf = 0.56) inhibited the tested bacteria with a weak potential category. Conclusion: Ethyl acetate extract of CFFB supernatant of Streptomyces G in ISP-4 media contained two different compounds and one of them showed growth inhibitory activity against Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 8739 based on the TLCbioautogram. Keywords: Streptomyces sp-G, compost, waste-soil, TLC-bioautography