Standardization of Eleutherine bulbosa Urb. Bulbs and Total Flavonoid Content from Three Locations in Kalimantan, Indonesia

Abstract

Background: Dayak Onion (Eleutherine bulbosa Urb.) is a typical plant of Kalimantan which is traditionally used by the Dayak community as a medicinal plant. Dayak onion bulbs have been proven had many pharmacology activities. Objective: This study aims to determine the nonspesific and specific parameters of 70% ethanol extract of Eleutherine bulbosa Urb. Total flavonoids was also quantified Methods:. Eleutherine bulbosa Urb was extracted with maseration method used etanol 70 % as solvent. Determination of non-spesific includes by determined specific gravity, water content, total ash content, acid insoluble ash content, residual sovents, heavy metanol contamination, microbial contamination, mold and yeast contamination. Determination of specific parameters included extract identity, organoleptic extract, water/ethanol soluble content, chromatography profile. Total flavonoid content were quantified with colorimetric method. Results: there were no significance difference between nonspesific and specific parameters Eleutherine bulbosa Urb from three different locations. Measurement of total phenol content and total flavonoid content respectively form South Borneo were ; 6,499 ± 0,5248 mg QE/g extract, from central borneo were 7,585 ± 0,0437 mgQE/g extract, and from east borneo were 5,035 mg ± 0,3887 mgQE/g extract. Conclusion: it can be concluded that bulbs of Eleutherine bulbosa Urb from three locations have characters to similar between each other and bulbs of Eleutherine bulbosa Urb form central borneo had the highest total flavonoid content. Key words: Eleutherine bulbosa Urb., Standardization, Non-specific parameters, Specific parameters, Flavonoids.