Tinospora Sinensis (Lour.) Merr. Stem Modulate The TNF-Alpha Expression In HCT- 116 Tumour Cell, Besides the Inhibitory Effect on Cervical, Colon and Breast Cancer Cell Lines and Mycobacterium Tuberculosis H37Rv

Abstract

The present study was designed to evaluate TNF-Alpha experession, anticancer and antitubercular properties for the stem extracts of Tinospora sinensis (TS). Objective: natural product research is widely used for identifying hit molecules for life threatening diseases including cancer, tuberculosis and drug resistant infections. Materials and Methods: There were three polarity dependant solvent extracts obtained through cold maceration process using ethanol (ELTS), ethyl acetate (EATS) and n-hexane (NHTS), respectively. The extracts were subjected to MTT assay for their anticancer potential against HeLa (cervical cancer), MCF-7 (breast cancer) and HCT116 (colon cancer) cell lines, and based on the results, NHTS was subjected to flow cytometry for TNF-Alpha expression in HCT-116 cells. The antitubercular activity for the extracts was performed against Mycobacterium tuberculosis H37Rv (Mtb) by luciferase reporter phage (LPS) assay method. Results: The result of anticancer screening revealed that n-hexane extracts showed the significant inhibition (p<0.05) on HCT-116 cells with the IC50 of 177.4 μg/ml, whereas EATS and ELTS were equally active on HeLa with the respective IC50 of 236 and 277 μg/ml. The NHTS was significantly effective on decreasing (P<0.05) TNF-Alpha expression (31.27 MFU) in HCT-116 cells and is closely active with standard simvastatin (26.7 MFU) against the control (7.06 MFU). The antitubercular activity results revealed the equi-potency of both NHTS and EATS on Mtb with growth inhibition of 84 % at 100μg/ml. The GC-MS analyses of NHTS confirmed the presence of Berberine, palmatine, tembertarine, magniflorine, choline and tinosporin. Conclusion: Overall, we scientifically support the traditional use Tinospora sinensis stem in the treatment of cancer and immune diseases. Key Words: Tinospora sinensis, Anticancer, Antitubercular, Immunomodulatory, TNF-Alpha, HCT-116.