In-vitro Sun Protecting Factor of Rice Bran Oil and Its Formulation as Compact Powder

Abstract

This study aims to determine the effective concentration of rice bran oil (RBO) which can protect against ultraviolet (UV) rays as well as formulate it in compact powder preparation followed by determination of its Sun Protecting Factor (SPF) value. Rice bran samples were extracted using the Soxhlet extraction method with n-hexane: ethanol (1:1) as the solvent. Further identification of the γ-oryzanol in RBO was carried out using TLC silica gel GF254 with eluent of n-hexane:ethyl acetate (3:1). The obtained γ-oryzanol in RBO was used as the ingredient to develop the compact powder which is made into five formulas with 0.05 %-0.25 % concentration. All formulas were characterized, including homogeneity, adhesion and crack test. UV-Visible spectrophotometry was used to determine the SPF value of RBO and its compact powder. The identification results demonstrated a positive presence of the chemical γ-oryzanol in the RBO. At concentrations of 500, 1000, 1500, 2000, and 2500 ppm, RBO may protect against UV rays with SPF values ranging from 1.741 to 11.884. The result showed that all formulas dispersed homogeneously, performed well in terms of compactness, and had no breaks or cracks discovered. Meanwhile, the SPF from five variation of compact powder formula showed value in the range of 1.390 - 2.178. The effective concentration of RBO from five concentration variations to protect against ultraviolet (UV) rays is 2500 ppm. However, the developed formulation compact powder using RBO 2000 ppm shows an SPF value that is sufficient to protect the skin from sun exposure which is included in shallow protection and are included in the minimal SPF category (2-4).