Profil Ketoksikan Akut SNEDDS Propolis

Abstract

ropolis has been widely published on its immunostimulant activity. Due to its poor solubility and bioavailability, nano formulation may resolve its problem. A study found that SNEDDS propolis enhanced its immunostimulant activity, but also raised its toxicity event. So, this study is designed to evaluate the acute toxicity effect on SNEDDS propolis. Using the standard method OECD 425, male Wistar rats were employed in this study. The assay started with a limit test (dose 2000 mg/kg BW) followed by a main test with starting dose of 175 mg/kg BW using factor 1.3. Intensive observation of toxic clinical signs was performed and continued by periodic observation for 14 days. At the end of the study, the rats were sacrificed and had their liver and kidneys for histopathological examination. Based on the result, there were no toxic clinical signs found nor reduction on animal body weight. The LD50 value was > 2000 mg/kg BW. There were no abnormalities on both histopathological observation of liver and kidneys. In conclusion, the SNEDDS propolis was not toxic on male Wistar rats (po).