Pengembangan Self-Nano Emulsifying System (SNES) Ekstrak Temulawak (Curcuma xanthorrhiza): Formulasi, Karakterisasi, dan Stabilitas

Abstract

The poorly-water soluble temulawak (Curcuma xanthorrhiza) extract has been widely studied and has potential for the treatment of diseases. Self-Nano Emulsifying System (SNES) is a method to increase the solubility of a poorly-water soluble extract by mixing it into a suitable vehicle. This research aimed to prepare, characterize, and test the stability of the SNES Curcuma xanthorrhiza extract. The selection of oil, surfactant, and cosurfactant components for screening of SNES formulation was determined by solubility study of Curcuma xanthorrhiza extract in various vehicles. Curcuma xanthorrhiza extract-loaded SNES was evaluated, including transmittance, particle size, polydisperse index (PI), zeta potential, thermodynamic stability, robustness to dilution, and accelerated stability test. The optimal formula for Curcuma xanthorrhiza extract-loaded-SNES was a combination of Labrasol (20%), Tween 20 (60%), and propyleneglycol (20%), with drug loading of Curcuma xanthorrhiza extract, was 23%. The characterization of Curcuma xanthorrhiza extract-loadedSNES showed that 100.1±0.0% of transmittance; 13.0±1.4 nm of particle size; 0.3±0.1 of PI; and-42.4±0.6 mV of zeta potential. The thermodynamic stability test demonstrated no phase separation. The robustness to the dilution showed that the particle size was stable during the dilution process. Curcuma xanthorrhiza extractloaded SNES was also stable during the accelerated stability test. Conclusion, Curcuma xanthorrhiza extract-loaded-SNES obtained a stable preparation with high drug loading.