Please use this identifier to cite or link to this item: http://localhost:8080/xmlui/handle/123456789/6992
Title: Desain Primer Gen 12S sRNA dari DNA Mitrokondria Babi (Sus scrofa) secara In Silico sebagai Kandidat Primer dalam Analisis Molekuler Kehalalan Produk
Authors: Fakih, Taufik Muhammad
Wijaya, Salsabilla
Priani, Sani Ega
Keywords: 12S rRNA gene
pork derivatives
Polymerase Chain Reaction (PCR)
primer candidate
in silico study
Issue Date: Dec-2021
Publisher: Jurnal Sains Farmasi & Klinis
Citation: Original Article
Abstract: Several products such as medicine, food, and cosmetics, especially collagen, can potentially contain pork derivatives, thus a halal analysis is needed. Polymerase Chain Reaction (PCR) is a method that can be used to perform molecular analysis of samples. This study aimed to design a primer candidate for the 12S rRNA pork gene through in silico. The method used was tracing the 12S rRNA gene data through the National Center for Biotechnology Information (NCBI) website, then the 12S rRNA gene sequence was analyzed using the Integrated DNA Technologies (IDT) webserver and MFEprimer-3.1 to select the best primer candidate. The selected primer candidates were then identified using the SnapGene Viewer webserver to observe the ability of the primer candidate to attach to the target sequence. In the last stage, the primer candidate evaluation is carried out using the OligoAnalyzerâ„¢ Tool webserver to obtain the best primer candidate pair that meets the good primer criteria. The best primer candidates are forward primer rRNA-5 (5' GTACTACTCGCAACTGCCTAAA 3') and reverse primer rRNA-6 (5'GCAAGGGTTGGTAAGGTCTATC 3') because they meet the ideal primer requirements. Therefore, these primer candidates can be used for in vitro sample characterization using the PCR technique.
URI: http://localhost:8080/xmlui/handle/123456789/6992
Appears in Collections:VOL 8 NO 3 2021

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