Penetapan Kadar Metformin dan Glimepiride secara Simultan pada Tablet Kombinasi Dosis Tetap yang dijual di Pasar Pramuka menggunakan Metode Spektrofotometri UV Tervalidasi

Abstract

Combination of metformin and glimepiride as a diabetes mellitus drug has recently shown significant increase due to its benefits in improving patient compliance and offering cost effectiveness. This study aimed at analyzing metformin-glimepiride in fixed dose combinations purchased at Pramuka Market (the largest drug market in Jakarta) using validated simultaneous UVspectrophotometry. The maximum wavelength of metformin and glimepiride is 237 nm and 228 nm respectively. The validation result of metformin and glimepiride at concentration range of 2-8 µg/mL and 5-17 µg/mL showed Linearity with 0.9999 and 0.9996, LOD values with 0.0962 µg/mL and 0.2954 µg/mL, LOQ values with 0.3208 µg/mL and 0.9846 µg/mL, and recovery (accuracy test) with 99.72% ± 1.88% to 103.86 ± 0.87%, respectively. The Relative Standard Deviation (RSD) as the precision test indicator for both drugs was less than 2%. The level of Metformin-glimepiride fixed-dose combination purchased at Pramuka Market was about 96.15% ± 3.56% to 102.71% ± 1.50%. The conclusion of this study showed that the level of metformin-glimepiride sold in the Pramuka market met the requirements.