Evaluation of Biochemical and Pathological Parameters at Different Doses of Cisplatin in Experimental Animal Model: Toxicological Study on an Antineoplastic Drug

Abstract

Evaluation of Biochemical and Pathological Parameters at Different Doses of Cisplatin in Experimental Animal Model: Toxicological Study on an Antineoplastic Drug Farhana Sultana1,2,3, Muhammed Mohibul Islam2, Mohammad Nurul Amin1,2,3, Nusrat Jahan2, Asma Kabir1,3, Talha Bin Emran4, Bibek Chandra Sutradhar5, Sujan Banik2* 1Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka 1229, Bangladesh 2Department of Pharmacy, Noakhali Science and Technology University, Noakhali 3815, Bangladesh 3Pratyasha Health Biomedical Research Center, Dhaka 1230, Bangladesh 4Department of Pharmacy, BGC Trust University Bangladesh, Chittagong 4381, Bangladesh 5Department of Medicine and Surgery, Chittagong Veterinary and Animal Science University, Chittagong 4225, Bangladesh Abstract Background: This study aimed to assess the effect of cisplatin-induced toxicities on biochemical and pathological parameters such as body, liver, and kidney weights, blood urea nitrogen (BUN), creatinine, alanine aminotransferase (ALT), and blood cells (RBCs and WBCs) in white Swiss albino mice. Methods: Cisplatin’s potential toxic effects on body, liver, and kidney weights were evaluated using standard laboratory methods. Blood biochemical levels such as BUN, creatinine, and ALT levels were determined by an auto-hemolyzer using commercial diagnostic kits. Blood cells (RBCs and WBCs) were counted under a microscope by a hemocytometer. Results: Cisplatin’s potential toxic effects on body, liver, and kidney weights were evaluated using standard laboratory methods. Blood biochemical levels such as BUN, creatinine, and ALT levels were determined by an auto-hemolyzer using commercial diagnostic kits. Blood cells (RBCs and WBCs) were counted under a microscope by a hemocytometer. Conclusions: This study suggested to increase caution when using cisplatin, particularly at high doses. Further investigation shall be performed to minimize its toxic effect and optimize its use. Keywords: alanine aminotransferase, biochemical parameters, blood urea nitrogen, cisplatin, mice