The effect of carvacrol on oxido-inflammatory ovarian injury and infertility induced by ischemia-reperfusion in rats

Abstract

The effect of carvacrol on oxido-inflammatory ovarian injury and infertility induced by ischemia-reperfusion in rats Nurullah Sahin1 , Ilhan Bahri Delibas2 , Unal Isaoglu3 , Bahadir Suleyman4 , Gulce Naz Yazici5 , Taha Abdulkadir Coban6 , Kemine Uzel7,*, Halis Suleyman4 , Veysel Arslan8 1Department of Gynecology and Obstetrics, Private Kastamonu Anadolu Hospital, 37200 Kastamonu, Turkey 2Department of Gynecology and Obstetrics, Faculty of Medicine, Tokat Gaziosmanpasa University, 60250 Tokat, Turkey 3Department of Gynecology and Obstetrics, Private Adatıp İstanbul Hospital, 34912 İstanbul, Turkey 4Department of Pharmacology, Faculty of Medicine, Erzincan Binali Yildirim University, 24100 Erzincan, Turkey 5Department of Pathology, Faculty of Medicine, Erzincan Binali Yildirim University, 24100 Erzincan, Turkey 6Department of Biochemistry, Faculty of Medicine, Erzincan Binali Yildirim University, 24100 Erzincan, Turkey 7Department of Obstetrics and Gynecology, Erzincan Binali Yıldırım University Mengucek Gazi Training and Research Hospital, 24000 Erzincan, Turkey 8Department of Bayburt Vocational High School of Health Services, Bayburt University, 69000 Bayburt, Turkey *Correspondence: kemineuzel@hotmail.com (Kemine Uzel) Academic Editor: Michael H. Dahan Submitted: 17 May 2021 Revised: 28 July 2021 Accepted: 11 August 2021 Published: 4 March 2022 Abstract Background: Increased oxidants and proinflammatory cytokines play a role in the pathogenesis of ovarian ischemia-reperfusion (OIR) injury and related infertility. Carvacrol has antioxidant, antibacterial and anti-inflammatory properties. Methods: Protective effect of carvacrol against ischemia-reperfusion (IR)-related ovarian damage and infertility was investigated. IR process were applied to the ovaries of rats, which were divided into the following groups (n = 12): OIR, IR +50 mg/kg carvacrol (IRC-50), IR +100 mg/kg carvacrol (IRC-100) and sham group (SG). After the reperfusion process, six rats from each group were killed and the removed ovaries were examined biochemically and histopathologically. The remaining animals were kept two months with mature male rats to reproduce. Results: At a dose of 50 mg/kg, carvacrol suppressed the oxidant parameter increase and antioxidant decrease caused by IR in ovarian tissue. At a dose of 100 mg/kg, carvacrol antagonized both oxidant and proinflammatory cytokine increase and antioxidant decrease. Histopathologically, severe degeneration of follicles in the ovaries of the OIR group, necrotic cell accumulations, hemorrhage in the corpus luteum, edema in the interstitial tissue, polymorphous nuclear leukocyte (PNL) infiltration, and congestion and dilation of blood vessels were detected. Inflammatory symptoms such as edema in the ovarian tissue, congested dilated blood vessels and PNL infiltration were observed at a dose of 50 mg/kg of carvacrol, but these histopathological findings were not observed at a dose of 100 mg/kg. Conclusion: A dose of 100 mg/kg carvacrol, which eliminated inflammatory damage, significantly prevented the development of IRinduced infertility. Carvacrol may be beneficial in the treatment of IR-related ovarian damage and infertility. Keywords: Carvacrol; Oxido-inflammatory; Infertility; Rat